Description
Specifications Table
Product Name – Puromycin Dihydrochloride Plant Culture Tested
Quantity/Pack Size – 10 mg
Form – White to off-white crystalline powder
Grade – Plant Culture Tested, Research Grade
Application – Selective plant cell culture, protein synthesis inhibition, genetic engineering applications
Product Overview
Puromycin Dihydrochloride is a powerful aminonucleoside antibiotic isolated from *Streptomyces alboniger*, widely recognized for its robust protein synthesis inhibitory capabilities. It functions by mimicking aminoacyl-tRNA, incorporating into nascent polypeptide chains during elongation, leading to premature termination of protein translation. This mechanism makes it an indispensable tool for molecular biologists and researchers studying gene expression and cellular processes. Our Puromycin Dihydrochloride is meticulously prepared and certified as Plant Culture Tested, ensuring optimal performance and minimal cytotoxic effects on plant cells, which is crucial for sensitive plant cell and tissue culture applications. Its high purity and consistent activity provide reliable selective pressure for plant cell transformation experiments, allowing for the isolation of successfully modified cells. Researchers rely on its efficacy for stable plant cell line development, ensuring the integrity and success of their genetic engineering projects. This product is a testament to quality, providing researchers with a trusted reagent for critical experiments demanding precise control over protein synthesis and efficient selection.
FAQs
1.
What is Puromycin Dihydrochloride?
Puromycin Dihydrochloride is an aminonucleoside antibiotic derived from *Streptomyces alboniger*, primarily known for its potent ability to inhibit protein synthesis in various biological systems.
2.
How does Puromycin Dihydrochloride function in plant cell culture?
In plant cell culture, Puromycin acts as a structural analog of tyrosyl-tRNA. It enters the A site of the ribosome and covalently binds to the nascent polypeptide chain, causing premature release and termination of protein synthesis. This allows for effective selection of genetically transformed plant cells that express a resistance gene.
3.
What are the recommended storage conditions for this product?
For optimal stability and longevity, Puromycin Dihydrochloride should be stored desiccated at -20°C. If reconstituted, avoid repeated freeze-thaw cycles to maintain its efficacy over time.
4.
Why is the “Plant Culture Tested” grade important for researchers?
The “Plant Culture Tested” grade signifies that this product has undergone rigorous validation to confirm its suitability and effectiveness specifically for plant cell and tissue culture applications. It ensures low phytotoxicity and reliable selective pressure for plant systems, which is critical for successful experiments.
5.
Is this product suitable for use in other types of cell culture beyond plants?
While this particular grade is optimized and validated for plant cell culture, Puromycin Dihydrochloride is also widely used for selection in mammalian cell culture due to its general mechanism of protein synthesis inhibition. Specific concentrations and protocols would need to be optimized for non-plant systems.
