Description
Specifications Table
Product Name – Pyronin Y For Molecular Biology C. I. No.: 45005
Quantity/Pack Size – 5 gm
Form – Powder
Grade – Molecular Biology Grade
Application – RNA/DNA Staining, Histology, Cytology, Fluorescent Microscopy
Product Overview
Pyronin Y is a vibrant red cationic xanthene dye highly valued in molecular biology and histological applications for its distinctive staining properties. With a C.I. No. of 45005, this high-purity reagent is particularly renowned for its ability to differentially stain RNA in the presence of DNA. When combined with methyl green, Pyronin Y forms a classic histological stain, where it selectively binds to RNA, rendering it red or pink, while methyl green stains DNA blue-green. This differential affinity makes Pyronin Y an indispensable tool for researchers and educators seeking to visualize and distinguish between nucleic acids within cellular structures. Its mechanism involves electrostatic interactions with the negatively charged phosphate backbone of nucleic acids, with a stronger affinity for the single-stranded nature of RNA compared to the double-stranded DNA. This property is crucial for analyzing cellular processes, identifying active ribosomes, and studying cytoplasmic RNA distribution. Beyond nucleic acid staining, Pyronin Y also finds use in vital staining, allowing for the observation of living cells without significant toxicity. It is also employed in fluorescence microscopy due to its fluorescent properties, making it valuable for dynamic cellular studies. The molecular biology grade ensures minimal impurities, providing consistent and reliable results for sensitive applications, making it suitable for a broad spectrum of cellular and tissue analysis. This versatile dye is a cornerstone reagent for detailed cellular analysis, supporting various research endeavors. Its consistent performance makes it a trusted choice for laboratories focused on accurate and reproducible biological staining outcomes.
FAQs
1. What is Pyronin Y primarily used for in molecular biology?
Pyronin Y is primarily used for the differential staining of RNA, often in conjunction with methyl green for distinguishing RNA from DNA in cells and tissues, making it valuable for cellular analysis.
2. How should Pyronin Y be stored to maintain its efficacy?
Pyronin Y should be stored in a cool, dry place, protected from direct light. Ensure the container is tightly sealed to prevent degradation and maintain its staining properties over time.
3. Is Pyronin Y compatible with fluorescence microscopy?
Yes, Pyronin Y exhibits fluorescent properties, making it suitable for applications in fluorescence microscopy to visualize and analyze cellular components that it stains.
4. Can Pyronin Y be used as a vital stain?
Yes, Pyronin Y can be used as a vital stain, allowing for the observation of living cells without causing significant immediate toxicity, although care should always be taken regarding concentrations and exposure times.
5. What grade of Pyronin Y is recommended for sensitive research?
For sensitive research applications, a molecular biology grade Pyronin Y, such as this product, is highly recommended to ensure high purity and minimal impurities for consistent and reliable experimental results.
