Description
Specifications Table
| Product Name | Streptavidin For Molecular Biology (Fibrinogen) |
|---|---|
| Pack Size | 5 mg |
| Form | Lyophilized Powder |
| Grade | Molecular Biology |
| Application | Biotin-binding assays, ELISA, Western Blot, Immunoprecipitation |
Product Description
Streptavidin For Molecular Biology (Fibrinogen) is a highly purified protein designed for researchers and students working in molecular biology, immunology, and biochemistry. With an activity of ~14 Units/mg protein, this streptavidin variant is optimized for high-affinity biotin binding, making it a reliable choice for applications like ELISA, Western blotting, and immunoprecipitation.
Whether you’re a student setting up your first biotin-streptavidin assay or a seasoned researcher refining your protocols, this product ensures consistent performance. The fibrinogen-free formulation minimizes non-specific binding, improving the accuracy of your results. At eqipped, we source this streptavidin from trusted manufacturers to guarantee quality and reproducibility in your experiments.
Looking to buy streptavidin online? This 5 mg pack is perfect for small-scale experiments or as a starter kit for new lab setups. Its stability and long shelf life make it a cost-effective choice for academic and research institutions across India.
Need high-purity streptavidin for your next experiment? Order now and get it delivered to your lab in just a few days.
Key Applications
This streptavidin is widely used in:
- Biotinylated protein detection in Western blots
- ELISA-based assays for quantitative analysis
- Immunoprecipitation of biotin-tagged molecules
- Cell surface labeling and flow cytometry
- DNA/RNA pull-down assays
Its high binding affinity (Kd ~10-15 M) ensures minimal dissociation, even under stringent washing conditions. Researchers often prefer this variant over avidin due to its lower non-specific binding and neutral pI, which reduces background noise in sensitive assays.
Why This Streptavidin Stands Out
Not all streptavidin products are created equal. This molecular biology-grade streptavidin is:
- Purified to remove fibrinogen contaminants, reducing interference in assays
- Tested for consistent biotin-binding activity (~14 Units/mg)
- Supplied in a lyophilized form for long-term stability
- Compatible with most buffer systems used in molecular biology
For students and educators, this product serves as an excellent teaching tool for demonstrating biotin-streptavidin interactions—a fundamental concept in modern biotechnology.
Storage and Handling
To maintain optimal activity, store the lyophilized streptavidin at -20°C. Once reconstituted in sterile water or buffer, aliquot and store at -20°C for up to 6 months. Avoid repeated freeze-thaw cycles to preserve protein integrity.
Pro tip: Always use nuclease-free water for reconstitution to prevent contamination in sensitive molecular biology applications.
Safety Information
This product is for research use only and is not intended for diagnostic or therapeutic applications. While streptavidin is generally non-toxic, standard lab safety practices should be followed:
- Wear appropriate PPE (gloves, lab coat, goggles)
- Avoid inhalation or ingestion
- Dispose of waste according to local regulations
FAQs
What is the difference between streptavidin and avidin?
Streptavidin is preferred in molecular biology due to its lower non-specific binding and neutral pI compared to avidin, which is derived from egg whites and has a higher isoelectric point.
Can this streptavidin be used for in vivo experiments?
No, this product is intended for in vitro research applications only. For in vivo use, specialized formulations with reduced immunogenicity are required.
How do I reconstitute the lyophilized streptavidin?
Add sterile distilled water or buffer (e.g., PBS) to achieve a concentration of 1 mg/mL. Gently mix without vortexing to avoid denaturation.
What is the shelf life of this product?
The lyophilized form is stable for up to 2 years when stored at -20°C. Reconstituted aliquots should be used within 6 months.
Is this streptavidin compatible with fluorescent labeling?
Yes, it can be conjugated with fluorescent dyes (e.g., FITC, Cy3) for imaging applications, though pre-conjugated versions may offer better performance for such uses.
