Description
Specifications Table
| Product Name | Tricine For Molecular Biology 99.0% | 989300 | CDH |
|---|---|
| Pack Size | 100 gm |
| Form | Powder |
| Grade | Molecular Biology |
| Application | Buffer reagent for protein electrophoresis, biochemical assays |
Product Description
Tricine, or N-[Tris(hydroxymethyl)methyl]glycine, is a high-purity buffer reagent widely used in molecular biology and biochemistry. With a purity of 99.0%, this molecular biology grade tricine is ideal for applications requiring precise pH control, such as protein electrophoresis, gel staining, and enzyme assays.
Unlike traditional buffers like glycine, tricine offers better resolution for low-molecular-weight proteins, making it a preferred choice in SDS-PAGE and other electrophoretic techniques. Its zwitterionic nature ensures minimal interference with protein mobility, providing sharper and more reliable results.
Whether you’re a student setting up a lab experiment or a researcher optimizing protein separation, this tricine buffer reagent delivers consistent performance. The 100 gm pack size is perfect for small to medium-scale applications, ensuring you have enough for multiple experiments without excess waste.
Need a reliable buffer for your molecular biology work? Buy tricine online from eqipped for guaranteed quality and fast shipping.
Tricine is also commonly used in conjunction with Tris and SDS buffers to create optimized running conditions for protein gels. Its compatibility with common staining methods like Coomassie Blue and silver staining makes it a versatile addition to any lab.
For best results, store tricine in a cool, dry place away from direct sunlight. Always handle with appropriate lab safety gear, as it is a fine powder that can cause irritation if inhaled or in contact with skin.
FAQs
What is the purity of this tricine?
This tricine is 99.0% pure, making it suitable for molecular biology applications where high purity is critical.
Can I use tricine for DNA electrophoresis?
While tricine is primarily used for protein electrophoresis, it can be used in DNA studies where a neutral, non-interfering buffer is needed.
How do I prepare a tricine buffer for SDS-PAGE?
A common recipe involves dissolving tricine in water, adjusting the pH with HCl or NaOH, and adding SDS. Refer to standard protocols for exact concentrations.
Is this tricine compatible with silver staining?
Yes, tricine is compatible with silver staining and other common protein detection methods.
What is the shelf life of tricine?
When stored properly in a cool, dry place, tricine has a long shelf life, typically several years. Always check for clumping or discoloration before use.
