Description
Specifications Table
Product Name – Xanthine Oxidase Cell Culture TestedActivity : ~15U/mg
Quantity/Pack Size – 250UNITS
Form – Lyophilized Powder
Grade – Cell Culture Tested, Activity: ~15U/mg
Application – Enzyme Assays, Purine Metabolism Studies, Oxidative Stress Research
Product Overview
Xanthine Oxidase, a pivotal enzyme in purine metabolism, is a crucial component for a wide array of biochemical and cellular research applications. Our Xanthine Oxidase is supplied as a high-purity preparation, rigorously tested to ensure it meets stringent Cell Culture Tested standards, making it exceptionally suitable for sensitive biological systems. With a specified activity of approximately 15U/mg, this enzyme guarantees consistent and reproducible results in your experiments. It catalyzes the oxidation of hypoxanthine to xanthine and subsequently xanthine to uric acid, playing a significant role in studies related to gout, oxidative stress, and various metabolic disorders. Researchers can rely on its superior quality for enzyme kinetics, inhibitor screening, and cellular pathway investigations. eqipped is committed to providing top-tier lab essentials, empowering students, researchers, and educators across India with the dependable tools they need for groundbreaking discoveries.
FAQs
1. What is the optimal storage condition for Xanthine Oxidase?
For maintaining its activity and stability, Xanthine Oxidase should ideally be stored at -20°C. Ensure the vial is tightly sealed to prevent moisture absorption.
2. Can this Xanthine Oxidase be used for in vitro diagnostic development?
While designed for research, the high purity and specified activity of this Xanthine Oxidase make it a suitable candidate for preliminary studies in in vitro diagnostic development, pending further validation for specific applications.
3. What is the typical activity unit definition for this enzyme?
One unit of Xanthine Oxidase typically catalyzes the oxidation of 1.0 µmole of hypoxanthine to uric acid per minute at pH 7.5 at 25°C.
4. How does the Cell Culture Tested grade benefit my experiments?
The Cell Culture Tested grade signifies that the product has undergone rigorous quality control to ensure it is free from contaminants that could interfere with cellular processes, providing reliable results for sensitive cell-based assays.
5. Are there any common cofactors or metal ions required for Xanthine Oxidase activity?
Xanthine Oxidase is a molybdoflavoenzyme containing molybdenum, iron-sulfur clusters, and FAD as cofactors essential for its catalytic activity. No additional cofactors are typically required beyond what is inherently part of the enzyme structure.
