Description
Specifications Table
Product Name – Decane sulfonic acid sodium salt, anhydrous HPLC
Quantity/Pack Size – 25 g
Form – Solid (powder/crystals)
Grade – HPLC (≥99% purity)
Application – Ion-pair reagent, chromatography, surfactant studies
Product Overview
Decane sulfonic acid sodium salt (anhydrous, HPLC grade) is a high-purity ionic modifier designed for demanding chromatographic applications where consistency and minimal interference are critical. This compound serves as an essential ion-pairing reagent in reversed-phase HPLC, enabling improved separation of polar or ionic analytes by forming neutral ion pairs that interact more effectively with nonpolar stationary phases. Its anhydrous form eliminates moisture-related variability, ensuring reproducible retention times and peak shapes across runs. The ≥99% purity level, confirmed via rigorous quality control, guarantees low baseline noise and negligible UV absorbance at common detection wavelengths (210–254 nm), making it ideal for sensitive analytical workflows. The sodium salt form enhances solubility in aqueous mobile phases while maintaining stability over extended storage periods when kept under recommended conditions. Its long hydrophobic chain (C10) provides optimal hydrophobic interactions for separating compounds with varying polarities, from small organic acids to peptides. Unlike technical-grade alternatives, this HPLC-certified variant undergoes additional purification steps to remove trace metals, residual solvents, and oxidative byproducts that could compromise data integrity. Compatible with most HPLC and UHPLC systems, it integrates seamlessly into existing methods without requiring extensive reoptimization.
FAQs
1. What is the shelf life of anhydrous decane sulfonic acid sodium salt when stored properly?
When stored in a tightly sealed container at room temperature (15–25°C) and protected from moisture, the product retains its specified purity for at least 24 months from the date of manufacture. Avoid exposure to humidity, as the anhydrous form can absorb moisture over time.
2. Can this be used as a direct replacement for heptane sulfonic acid sodium salt in existing HPLC methods?
While both are ion-pairing reagents, decane sulfonic acid (C10) offers stronger hydrophobic interactions compared to heptane (C7), which may alter retention times. Method revalidation is recommended, starting with a 10–20% lower concentration than the heptane equivalent to account for the increased chain length.
3. What mobile phase pH range is compatible with this reagent?
The reagent remains stable and effective across a pH range of 2.0–7.5 in aqueous mobile phases. Below pH 2.0, protonation of the sulfonate group may reduce ion-pairing efficiency, while above pH 7.5, precipitation risks increase, especially in the presence of divalent cations.
4. Are there any known incompatibilities with common HPLC column stationary phases?
This reagent is compatible with most reversed-phase columns (C18, C8, phenyl) and hybrid particles (e.g., BEH, core-shell). However, prolonged use with bare silica or normal-phase columns may lead to irreversible adsorption due to the ionic nature of the sulfonic acid group.
5. How does the anhydrous form differ from the hydrated version in performance?
The anhydrous form eliminates water content variability, which can affect reagent concentration and ion-pairing strength in mobile phases. Hydrated versions may introduce inconsistent baseline shifts in gradient methods, whereas the anhydrous grade ensures batch-to-batch reproducibility in retention times and peak areas.
