Description
Specifications Table
Product Name – Ammonium acetate HPLC
Quantity/Pack Size – 500 g
Form – Crystalline powder
Grade – HPLC (≥98% purity)
Application – Chromatography, mass spectrometry, buffer preparation
Product Overview
Ammonium acetate HPLC grade is a high-purity, crystalline powder designed for demanding laboratory applications where precision and consistency are critical. With a minimum assay of 98%, this reagent ensures reliable performance in high-performance liquid chromatography (HPLC), mass spectrometry, and other analytical techniques. Its low-endotoxin content minimizes interference, making it suitable for sensitive assays and research workflows. The compound exhibits excellent solubility in water and polar organic solvents, allowing seamless integration into buffer systems and mobile phases. Unlike lower-grade alternatives, this HPLC-grade ammonium acetate undergoes rigorous quality control to eliminate trace impurities, ensuring reproducible results in quantitative and qualitative analyses. Its neutral pH in solution further enhances compatibility with a wide range of analytical instruments and sample types. Whether used as a volatile buffer in LC-MS or as a reagent in protein precipitation, this product delivers the purity and performance required for advanced laboratory protocols.
FAQs
1. What is the difference between HPLC-grade and reagent-grade ammonium acetate?
HPLC-grade ammonium acetate undergoes additional purification steps to remove trace metals, organic impurities, and endotoxins that could interfere with chromatographic separations or mass spectrometry detection. Reagent-grade versions may contain higher levels of contaminants, which can affect baseline stability and peak resolution in sensitive applications.
2. Can this product be used for LC-MS applications without further purification?
Yes, this HPLC-grade ammonium acetate is specifically formulated for direct use in LC-MS applications. Its low impurity profile minimizes ion suppression and background noise, ensuring accurate mass spectral data without requiring additional purification steps.
3. How should this product be stored to maintain its stability?
Store the unopened container in a cool, dry place away from direct sunlight and moisture. After opening, tightly reseal the container and use within 12 months for optimal performance. Avoid exposure to humid environments, as ammonium acetate is hygroscopic and may absorb moisture over time.
4. Is this ammonium acetate compatible with reverse-phase HPLC columns?
Yes, this product is fully compatible with reverse-phase HPLC columns when used at appropriate concentrations (typically 5–50 mM). Its volatile nature allows for easy removal during sample preparation, reducing column contamination and extending column lifespan.
5. What alternatives exist if I need a lower-purity ammonium acetate for general lab use?
For non-chromatographic applications such as general buffer preparation or educational experiments, reagent-grade or ACS-grade ammonium acetate can be used. These grades are more cost-effective but may contain higher levels of impurities that could impact sensitive analytical techniques.
