Description
Specifications Table
Product Name – Carbol Fuchsin solution (Ziehl-Neelsen) Indicator
Quantity/Pack Size – 125 ml
Form – Liquid
Grade – Laboratory Reagent
Application – Acid-fast staining (Ziehl-Neelsen method)
Product Overview
Carbol Fuchsin solution is a specialized staining reagent designed for the Ziehl-Neelsen method, widely used in microbiology for detecting acid-fast bacilli such as Mycobacterium tuberculosis. This concentrated solution combines basic fuchsin dye with phenol (carbolic acid) to enhance penetration and retention in waxy bacterial cell walls, ensuring clear differentiation under microscopic examination. The formulation is optimized for consistent staining intensity, reducing background interference and improving diagnostic accuracy. As a laboratory-grade reagent, it adheres to strict quality standards, guaranteeing reliability in routine and research applications. The solution is stable under standard storage conditions, maintaining its efficacy over extended periods. Its deep red color provides high contrast against counterstains like methylene blue, facilitating easy identification of target organisms. Whether used in clinical or educational settings, this Carbol Fuchsin solution delivers reproducible results, making it an essential tool for microbiologists and pathologists.
FAQs
1. What is the shelf life of Carbol Fuchsin solution when stored properly?
The solution remains stable for 24 months from the date of manufacture when stored in a tightly sealed container at room temperature, away from direct sunlight and moisture.
2. Is this Carbol Fuchsin solution compatible with automated staining systems?
Yes, the solution is formulated to work with both manual and automated staining protocols, though optimization may be required for specific automated platforms to achieve optimal results.
3. Can this solution be diluted for use, and if so, what is the recommended dilution ratio?
The concentrated solution is typically diluted 1:10 with distilled water for standard Ziehl-Neelsen staining, though the ratio may vary based on specific laboratory protocols or sample types.
4. What safety precautions should be taken while handling Carbol Fuchsin solution?
Wear appropriate personal protective equipment, including gloves and goggles, as the solution contains phenol, which can cause skin and eye irritation. Work in a well-ventilated area or under a fume hood.
5. How does the staining performance compare to other acid-fast stains like Kinyoun’s method?
Carbol Fuchsin in the Ziehl-Neelsen method uses heat to enhance staining, making it more effective for waxy cell walls compared to Kinyoun’s cold staining technique, which may require longer exposure times.
