Description
Specifications Table
Product Name – N,O-Bis(trimethylsilyl)acetamide
Quantity/Pack Size – 100 ml
Form – Liquid
Grade – Reagent Grade
Application – GC-MS derivatization, silylation of alcohols/phenols
Product Overview
N,O-Bis(trimethylsilyl)acetamide (BSA) is a high-purity silylating reagent designed for gas chromatography-mass spectrometry (GC-MS) derivatization processes. This colorless liquid reagent offers exceptional reactivity with active hydrogen compounds, including alcohols, phenols, and carboxylic acids, converting them into volatile trimethylsilyl (TMS) derivatives for enhanced chromatographic separation and detection. With a minimum assay of 98%, this reagent-grade BSA ensures reliable and reproducible results in analytical applications. Its low moisture content (<0.1%) prevents premature hydrolysis, maintaining stability during storage and use. The reagent's boiling point of 70-72°C at 12 mmHg facilitates efficient derivatization under controlled conditions. BSA is particularly effective for analyzing polar compounds that would otherwise be unsuitable for direct GC-MS analysis due to their thermal instability or low volatility. The reagent's compatibility with most organic solvents allows for flexible sample preparation protocols. Its high silylation efficiency reduces the need for excessive reagent quantities, making it cost-effective for routine laboratory use while minimizing potential interference in mass spectral analysis.
FAQs
1. What is the shelf life of N,O-Bis(trimethylsilyl)acetamide when stored properly?
When stored in a tightly sealed container under inert gas at 2-8°C, this reagent maintains its specified purity for at least 24 months from the date of manufacture. Always check the container label for the exact expiration date.
2. Can this BSA reagent be used for derivatizing amino acids?
While primarily used for alcohols and phenols, this reagent can derivatize amino acids, though complete silylation may require additional reaction time or temperature optimization compared to dedicated amino acid derivatization reagents.
3. What safety precautions should be taken when handling this silylating agent?
This reagent should be handled in a fume hood due to its moisture sensitivity and potential to release trimethylsilanol vapors. Use appropriate PPE including nitrile gloves, safety goggles, and lab coat, as it can cause skin and eye irritation.
4. How does this BSA compare to BSTFA for derivatization applications?
BSA generally offers faster reaction times for alcohols and phenols compared to BSTFA, though BSTFA may provide better results for amines. The choice depends on your specific analyte profile and desired reaction conditions.
5. What’s the recommended storage condition after opening the container?
After opening, store the reagent in a desiccator or under dry nitrogen at 2-8°C. Use a septum-sealed bottle if available to minimize moisture exposure during repeated access.
