Description
Specifications Table
Product Name – Cesium chloride Ultrapure Bioreagent
Quantity/Pack Size – 100 g
Form – Crystalline powder
Grade – Ultrapure Bioreagent (ACS)
Application – Density gradient centrifugation, nucleic acid purification
Product Overview
Cesium chloride Ultrapure Bioreagent is a high-purity, ACS-grade crystalline powder designed for critical laboratory applications requiring precision and consistency. With its exceptional solubility in water and low impurity profile, this reagent is ideal for density gradient centrifugation, where its ability to form stable gradients is essential for separating nucleic acids, proteins, and subcellular components. The ultrapure grade ensures minimal interference in downstream applications, making it a preferred choice for molecular biology workflows. Its hygroscopic nature necessitates proper storage to maintain purity, while its high density (3.989 g/cm³ at 25°C) enables effective separation of biomolecules based on buoyant density. Compatible with most aqueous buffers, this reagent dissolves rapidly without residue, ensuring reproducibility in experimental protocols. Whether used in analytical or preparative scales, its consistent performance reduces variability, enhancing the reliability of research outcomes. The absence of heavy metal contaminants and other impurities further ensures compatibility with sensitive assays, including PCR and sequencing preparations.
FAQs
1. What is the solubility of cesium chloride in water at room temperature?
Cesium chloride is highly soluble in water, with a solubility of approximately 186 g/100 mL at 20°C, forming clear, colorless solutions.
2. Can this grade be used for protein purification alongside nucleic acid isolation?
Yes, the ultrapure bioreagent grade is suitable for both nucleic acid and protein purification due to its low impurity levels and consistent density gradient formation.
3. How should cesium chloride be stored to prevent contamination?
Store in a tightly sealed container in a cool, dry place, protected from moisture and light to prevent hygroscopic absorption and degradation.
4. Is this product compatible with ethanol precipitation methods?
While cesium chloride itself is soluble in water, it is not typically used in ethanol precipitation; however, it can be removed via dialysis or centrifugation prior to ethanol-based steps.
5. What alternatives exist for density gradient centrifugation if cesium chloride is unavailable?
Alternatives include iodixanol (OptiPrep) or sucrose gradients, though these may require protocol adjustments due to differences in density and viscosity.
