Description
Specifications Table
Product Name – Chloroform, hydrocarbon stabilized HPLC
Quantity/Pack Size – 4 L
Form – Liquid
Grade – HPLC
Application – Chromatography, solvent extraction, analytical testing
Product Overview
This HPLC-grade chloroform is hydrocarbon-stabilized to prevent degradation and ensure consistent performance in sensitive analytical applications. With a minimum purity of 99.8%, it meets stringent requirements for chromatography, including low UV absorbance at 245 nm (<0.01 AU) and minimal residual impurities. The hydrocarbon stabilization (typically with amylene or ethanol) extends shelf life while maintaining compatibility with most lab instruments. Its low water content (<0.01%) and absence of heavy metals make it suitable for trace analysis and sample preparation. The solvent is packaged in amber glass bottles to prevent photodegradation, with tamper-evident seals for quality assurance. Its controlled evaporation rate and chemical stability provide reproducible results across multiple experimental runs. The product complies with ACS and Reag. Ph Eur specifications, ensuring reliability for research-grade applications where solvent purity is critical.
FAQs
1. Can this chloroform be used for DNA extraction protocols?
Yes, this HPLC-grade chloroform is suitable for nucleic acid extraction due to its high purity and low levels of nucleases or other contaminants that could degrade DNA/RNA samples.
2. What is the typical shelf life of hydrocarbon-stabilized chloroform?
When stored properly in tightly sealed original containers at room temperature, this stabilized chloroform maintains its specifications for 24 months from the date of manufacture.
3. How does hydrocarbon stabilization affect the solvent’s properties?
The hydrocarbon additive (usually 0.5-1% amylene or ethanol) prevents phosgene formation from UV degradation without significantly altering the solvent’s polarity or chromatographic behavior.
4. Is this chloroform compatible with plastic containers for storage?
While short-term contact with HDPE or PTFE is acceptable, prolonged storage should always be in the original glass container as chloroform can permeate most plastics over time.
5. What safety precautions should be taken when handling this product?
Always use in a well-ventilated fume hood, wear appropriate PPE (nitrile gloves, safety goggles), and avoid inhalation as chloroform vapors can cause dizziness or unconsciousness at high concentrations.
