Description
Specifications Table
Product Name – Trifluoroacetic acid HiPerSolv CHROMANORM® for LC-MS
Quantity/Pack Size – 100 ml
Form – Liquid
Grade – HiPerSolv CHROMANORM® (LC-MS Grade)
Application – LC-MS, HPLC, Mass Spectrometry
Product Overview
Trifluoroacetic acid (TFA) HiPerSolv CHROMANORM® is a high-purity reagent specifically formulated for liquid chromatography-mass spectrometry (LC-MS) applications. This ultra-pure grade ensures minimal background interference, making it ideal for sensitive analytical techniques where reproducibility and accuracy are critical. The HiPerSolv CHROMANORM® certification guarantees low levels of impurities, including metals, non-volatile residues, and UV-absorbing compounds, which can otherwise compromise LC-MS performance. Its strong acidic properties (pKa ~0.23) make it an effective ion-pairing agent for reverse-phase chromatography, enhancing peptide and protein separation. The liquid form allows for easy integration into mobile phases without additional preparation. Compatible with most LC-MS systems, this TFA variant maintains instrument longevity by reducing column contamination risks. Its volatility ensures efficient evaporation during sample preparation, minimizing carryover effects in subsequent runs. The 100 ml packaging provides an optimal balance between laboratory convenience and storage efficiency, with a shelf life of 24 months when stored under recommended conditions.
FAQs
1. What is the purity level of this TFA compared to standard reagent grades?
This HiPerSolv CHROMANORM® grade TFA undergoes additional purification steps to remove trace metals and UV-active impurities that are typically present in standard reagent grades. The specification limits for non-volatile residues are typically below 5 ppm, compared to 10-50 ppm in standard grades.
2. Can this TFA be used with stainless steel LC-MS systems?
While compatible with most LC-MS systems, prolonged exposure to TFA at concentrations above 0.1% may cause corrosion in stainless steel components over time. For long-term use, consider passivated systems or lower concentrations where possible.
3. What are the recommended storage conditions to maintain stability?
Store unopened bottles at 15-25°C in a well-ventilated area away from direct sunlight and incompatible materials. After opening, tightly reseal the container and use within 12 months for optimal performance.
4. How does this TFA compare to formic acid for LC-MS applications?
TFA provides stronger ion-pairing capabilities than formic acid, particularly for hydrophobic peptides, but may suppress ionization more significantly in ESI sources. Formic acid generally offers better sensitivity for small molecules but less retention for peptides.
5. What safety precautions should be taken when handling this product?
Always use in a fume hood with proper PPE including acid-resistant gloves and goggles. The vapor pressure of 117 mmHg at 20°C requires adequate ventilation. Neutralize spills with sodium bicarbonate solution before cleanup.
