Description
Specifications Table
Product Name – Ninhydrin, spectrophotometric grade
Quantity/Pack Size – 25GR, 100GR, 500GR
Form – Crystalline powder
Grade – Spectrophotometric
Application – Amino acid detection, peptide analysis
Product Overview
Ninhydrin, spectrophotometric grade, is a highly purified reagent designed for precise amino acid and peptide analysis. This crystalline powder exhibits exceptional clarity and consistency, ensuring accurate spectrophotometric measurements with minimal interference. With an assay purity of 98% or higher, it delivers reliable results in colorimetric assays, where even trace impurities can compromise data integrity. The reagent reacts with free amino groups to produce a characteristic blue-purple complex (Ruhemann’s purple), enabling quantitative detection at 570 nm. Its low moisture content and stable formulation prevent degradation during storage, maintaining performance over extended periods. The fine particle size ensures rapid dissolution in aqueous and organic solvents, facilitating seamless integration into existing protocols. Unlike technical-grade alternatives, this spectrophotometric-grade variant undergoes rigorous quality control to eliminate metallic and organic contaminants that could skew absorbance readings. Whether used in manual assays or automated systems, its consistent reactivity and high sensitivity make it indispensable for laboratories prioritizing reproducibility. The product is packaged in airtight containers to preserve purity and prevent hydration, with batch-specific certificates of analysis provided for traceability.
FAQs
1. What solvents are compatible with this spectrophotometric-grade ninhydrin?
It dissolves readily in ethanol, methanol, dimethyl sulfoxide (DMSO), and aqueous buffers at pH 4-8. Avoid prolonged exposure to strongly acidic or alkaline solutions to prevent decomposition.
2. How does this grade differ from reagent-grade ninhydrin?
Spectrophotometric grade undergoes additional purification to remove UV-absorbing impurities, ensuring baseline stability in absorbance measurements. Reagent grade may contain trace metals or organics that interfere with sensitivity.
3. What is the typical shelf life when stored properly?
When kept in the original sealed container at 2-8°C and protected from light, the reagent remains stable for 24 months. Hygroscopic nature requires desiccant use after opening.
4. Can this be used for post-column derivatization in HPLC?
Yes, its high purity and consistent reactivity make it suitable for HPLC applications. Pre-filter through 0.22 µm membranes to avoid particulate interference in chromatographic systems.
5. What safety precautions should be followed during handling?
Wear nitrile gloves, safety goggles, and work in a fume hood. The powder is irritating to skin and respiratory tract; avoid inhalation. Neutralize spills with sodium bicarbonate solution before disposal.
