Description
Specifications Table
Product Name – TAPS, 99+%, for biochemistry
Quantity/Pack Size – 25GR, 100GR
Form – Powder
Grade – 99%+
Application – Biochemistry buffer, protein studies, electrophoresis
Product Overview
TAPS (N-Tris(hydroxymethyl)methyl-3-aminopropanesulfonic acid) is a high-purity zwitterionic buffer with exceptional performance in biochemical applications. With a purity of 99%+, this reagent ensures minimal interference in sensitive assays, making it ideal for protein studies, electrophoresis, and other pH-critical experiments. Its pKa of 8.4 at 25°C provides excellent buffering capacity in the alkaline range (7.7–9.1), maintaining stable pH even under varying temperatures and ionic strengths. The powder form allows for easy preparation of concentrated stock solutions, while its low reactivity with metal ions and compatibility with most biochemical systems enhance its versatility. Unlike traditional buffers, TAPS exhibits minimal UV absorbance, ensuring accurate spectrophotometric measurements. Its non-toxic, non-volatile nature further simplifies handling and disposal in laboratory settings.
FAQs
1.
What is the ideal pH range for TAPS buffer?
TAPS functions optimally between pH 7.7–9.1, with a pKa of 8.4 at 25°C, making it suitable for alkaline-sensitive applications. 2.
Can TAPS be used with divalent metal ions?
Yes, TAPS has low chelation properties, allowing compatibility with most metal ions commonly used in biochemical assays. 3.
How should TAPS powder be stored for long-term use?
Store in a cool, dry place in a tightly sealed container to prevent moisture absorption and degradation. 4.
Is TAPS compatible with reducing agents like DTT?
TAPS remains stable in the presence of reducing agents, making it suitable for protein denaturation and refolding studies. 5.
What alternatives exist for TAPS in high-salt buffers?
HEPES or CHAPS can be considered, but TAPS offers better alkaline stability and lower UV interference.
