Specifications Table
Product Name – Trifluoroacetic acid, 99+%, for HPLC
Quantity/Pack Size – 100ML, 500ML
Form – Liquid
Grade – HPLC
Application – Chromatography, peptide synthesis, protein sequencing
Product Overview
Trifluoroacetic acid (TFA) at 99% purity is a high-performance reagent specifically designed for HPLC (High-Performance Liquid Chromatography) applications, where precision and reproducibility are critical. This grade ensures minimal interference from impurities, making it ideal for sensitive analytical techniques. Its strong acidic nature (pKa ~0.23) and volatility allow for efficient solvent compatibility, particularly in reverse-phase chromatography and mass spectrometry. The compound’s ability to ionize analytes enhances peak resolution, while its low UV absorbance minimizes background noise. TFA is also widely used in peptide synthesis as a cleavage reagent and in protein sequencing due to its ability to solubilize hydrophobic peptides without causing significant degradation. The liquid form ensures easy handling and dosing, while its stability under standard lab conditions guarantees consistent performance. For laboratories requiring reliable, high-purity acids for chromatographic separations or biochemical applications, this HPLC-grade TFA delivers exceptional clarity and reproducibility, reducing the need for additional purification steps.
FAQs
1. What is the shelf life of HPLC-grade trifluoroacetic acid?
When stored in a tightly sealed container at room temperature, away from moisture and direct sunlight, this product retains its purity and effectiveness for up to 24 months from the date of manufacture.
2. Can this TFA be used for LC-MS applications?
Yes, this 99% pure trifluoroacetic acid is compatible with LC-MS due to its low impurity profile, which minimizes ion suppression and background noise during mass spectrometric detection.
3. Is this product compatible with glass or plastic containers?
It is recommended to store and handle this acid in glass containers, as TFA can degrade certain plastics over time, leading to contamination or container failure.
4. What precautions should be taken while handling trifluoroacetic acid?
Always use personal protective equipment, including gloves, goggles, and a lab coat, as TFA is highly corrosive. Work in a fume hood to avoid inhalation of vapors, and neutralize spills immediately with a suitable base like sodium bicarbonate.
5. Are there any alternatives to TFA for HPLC mobile phase modification?
Formic acid is a common alternative for LC-MS applications where TFA’s ion-pairing effects are undesirable, though it may offer different selectivity and retention characteristics.