Specifications Table

Product Name – Tris(hydroxymethyl)aminomethane, 99.8%, ACS reagent
Quantity/Pack Size – 100GR, 500GR, 2.5KG
Form – Crystalline powder
Grade – ACS reagent
Application – Buffering agent, pH regulation

Product Overview

Tris(hydroxymethyl)aminomethane, commonly known as Tris buffer, is a highly purified (99.8%) ACS-grade reagent designed for precise pH control in laboratory applications. This crystalline powder is widely recognized for its exceptional buffering capacity, particularly in the pH range of 7.0 to 9.2, making it indispensable for biochemical and molecular biology protocols. The ACS-grade certification ensures minimal impurities, guaranteeing consistent performance in sensitive assays. Its high solubility in water and low reactivity with most biological molecules further enhance its utility. The compound’s stable chemical structure prevents pH drift, even under varying temperatures, ensuring reliable results in critical experiments. Whether used in protein purification, nucleic acid extraction, or enzyme reactions, this Tris buffer maintains optimal conditions for reproducible outcomes. The reagent’s versatility extends to compatibility with common lab equipment and protocols, reducing the need for additional adjustments. With its well-documented stability and purity, this product is a trusted choice for researchers demanding accuracy and reproducibility in their work.

FAQs

1. What is the ideal pH range for Tris buffer?

Tris buffer is most effective in the pH range of 7.0 to 9.2, making it suitable for a wide range of biochemical applications where stable alkaline conditions are required.

2. Can this Tris buffer be used for cell culture applications?

While primarily used in biochemical assays, this ACS-grade Tris buffer can be employed in cell culture media preparation, provided it is sterilized and adjusted to the required pH.

3. How should this product be stored to maintain its purity?

Store the Tris buffer in a cool, dry place, tightly sealed in its original container to prevent moisture absorption and contamination.

4. Is this Tris buffer compatible with EDTA or other chelating agents?

Yes, Tris buffer can be used alongside EDTA and other chelating agents, but compatibility should be verified for specific applications to avoid precipitation or interference.

5. What alternatives exist if Tris buffer is not suitable for my application?

Alternatives like HEPES or phosphate buffers can be considered, depending on the required pH range and experimental conditions.