Specifications Table

Product Name – Water, for molecular biology, DNAse, RNAse and Protease free
Quantity/Pack Size – 1LT, 5LT
Form – Liquid
Grade – Molecular Biology Grade
Application – PCR, qPCR, sequencing, cloning, nucleic acid purification

Product Overview

This ultrapure water is specifically formulated for critical molecular biology applications where even trace contaminants can compromise results. Rigorously tested to be free of DNAse, RNAse, and protease activity, it ensures the integrity of nucleic acids and proteins during sensitive procedures. The water undergoes a multi-stage purification process, including reverse osmosis, deionization, and 0.1 µm filtration, followed by autoclaving to eliminate endotoxins and microbial contaminants. Its low conductivity (<1 µS/cm) and resistance (18.2 MΩ·cm at 25°C) guarantee minimal ionic interference, making it suitable for downstream applications like enzyme reactions, buffer preparation, and reagent dilution. The packaging in sterile, pyrogen-free containers prevents secondary contamination, while the lot-specific certification provides traceability for reproducible experiments. Unlike standard laboratory-grade water, this product meets the stringent requirements of ISO 3696 Type I standards, ensuring consistency across batches for high-throughput workflows.

FAQs

1. Is this water autoclaved, and can it be used directly for cell culture?

It is sterile-filtered and endotoxin-tested but not supplemented with nutrients or buffers required for cell culture. For mammalian cell applications, use cell culture-grade water with added supplements.

2. How does this differ from regular distilled or deionized water?

Regular distilled or deionized water lacks certification for nuclease/protease absence and may contain trace organic contaminants, endotoxins, or particulate matter that interfere with molecular assays.

3. What is the shelf life, and how should it be stored after opening?

Unopened bottles have a 24-month shelf life. After opening, store at room temperature (15–25°C) and use within 3 months to prevent microbial contamination. Avoid repeated exposure to air.

4. Can this water be used for preparing buffers or diluting enzymes?

Yes, its ultra-low ionic content and absence of nucleases/proteases make it ideal for buffer preparation, enzyme dilution, and reagent reconstitution without affecting activity.

5. Does it contain any additives like DEPC or other stabilizers?

No, this water is free from DEPC, azides, or other chemical additives. Its purity is maintained through physical purification methods only.