Description
Specifications Table
Product Name – Digitonin 99% AR
Quantity/Pack Size – 1 g
Form – Powder
Grade – Analytical Reagent (AR)
Application – Cell permeabilization, cholesterol research, biochemical assays
Product Overview
Digitonin 99% AR is a high-purity, plant-derived saponin widely used in biochemical and cell biology research. This analytical reagent-grade compound is known for its selective permeabilization of cell membranes, making it invaluable for studying intracellular components without compromising structural integrity. Its high purity (99%) ensures minimal interference in sensitive assays, particularly those involving cholesterol interactions or membrane protein analysis. The powder form allows for precise dosing and easy dissolution in aqueous solutions, maintaining stability under standard lab conditions. Unlike crude saponin extracts, this AR-grade digitonin undergoes rigorous purification to remove impurities, ensuring consistent performance in critical applications. Researchers rely on its ability to create stable pores in cholesterol-rich membranes while leaving other cellular structures intact. The compound’s well-defined chemical structure and batch-to-batch consistency make it a preferred choice for reproducible experimental results in membrane biochemistry and related fields.
FAQs
1. What is the solubility profile of Digitonin 99% AR in common lab solvents?
Digitonin 99% AR is soluble in water and methanol, forming clear to slightly turbid solutions depending on concentration. It has limited solubility in ethanol and is practically insoluble in non-polar solvents like chloroform or hexane.
2. How does the AR grade differ from technical-grade digitonin?
The AR grade undergoes additional purification steps to achieve 99% purity, with strictly controlled levels of impurities like heavy metals and other saponins. Technical-grade digitonin typically contains 50-70% active compound with higher variability between batches.
3. What storage conditions maximize the shelf life of this product?
Store Digitonin 99% AR in a tightly sealed container at 2-8°C, protected from light and moisture. Under these conditions, the product remains stable for at least 24 months from the date of manufacture.
4. Can this digitonin be used for plant cell membrane studies?
While primarily used for animal cell membranes, Digitonin 99% AR can permeabilize plant cell membranes, though its effectiveness varies with plant species due to differences in membrane sterol composition.
5. What safety precautions should be taken when handling this compound?
Wear appropriate PPE including gloves, goggles, and lab coat. Avoid inhalation of dust and work in a well-ventilated area or fume hood, as digitonin can cause irritation to skin, eyes, and respiratory tract.
