Description

Specifications Table

Product Name – AGAROSE (L) For Electrophoresis

Quantity/Pack Size – 10 g, 25 g, 100 g

Form – Powder

Grade – Molecular Biology (Low EEO)

Application – DNA/RNA electrophoresis, nucleic acid separation

Product Overview

AGAROSE (L) is a high-purity, low electroendosmosis (EEO) agarose specifically formulated for nucleic acid electrophoresis. Engineered with controlled pore size, it delivers exceptional gel strength and clarity, ensuring sharp band resolution for DNA and RNA fragments. The low EEO property minimizes background interference, enhancing the accuracy of molecular weight determination. This agarose is free from DNase, RNase, and protease contaminants, making it suitable for sensitive applications requiring intact nucleic acids. Its consistent gelation properties allow for reproducible results across experiments. The powder form dissolves uniformly in standard electrophoresis buffers, forming stable gels that withstand prolonged runs without sagging or cracking. Optimized for both analytical and preparative electrophoresis, this agarose maintains performance across a wide range of voltages and buffer systems. Its neutral pH ensures compatibility with downstream applications like blotting or elution.

FAQs

1.

This low-EEO agarose offers 1.2-1.5% higher gel strength than standard agarose at equivalent concentrations, providing better structural integrity for high-resolution separations.

2.

Yes, it is fully compatible with both TAE and TBE buffers, though gel strength may vary slightly depending on buffer composition and pH.

3.

The melting temperature is approximately 88-90°C, while the gelling temperature ranges between 36-38°C at 1% concentration.

4.

While ethidium bromide is commonly used, this agarose is also compatible with alternative DNA stains like SYBR Safe or GelRed for safer visualization.

5.

Store the unopened container in a cool, dry place (15-30°C) away from direct sunlight. Reseal the pack tightly after each use to prevent moisture absorption.