Description

Specifications Table

Product Name – AGAROSE (H) For Electrophoresis
Quantity/Pack Size – 10 g, 25 g, 100 g
Form – Powder
Grade – Molecular Biology Grade
Application – DNA/RNA electrophoresis, nucleic acid separation

Product Overview

AGAROSE (H) is a high-purity, low-electroendosmosis (EEO) agarose specifically formulated for molecular biology applications requiring superior nucleic acid separation. Engineered with consistent gel strength and minimal background interference, this agarose delivers sharp, well-defined bands during horizontal gel electrophoresis. The optimized pore size ensures efficient resolution of DNA fragments ranging from 50 bp to 20 kb, making it suitable for both analytical and preparative procedures. Its molecular biology grade certification guarantees absence of DNase, RNase, and other contaminating enzymes that could compromise experimental integrity. The powder form allows for precise gel concentration adjustments (typically 0.7–2.0%) to accommodate different fragment sizes. AGAROSE (H) exhibits excellent clarity post-staining with ethidium bromide or other nucleic acid dyes, facilitating accurate visualization under UV transillumination. The product undergoes rigorous quality control to maintain batch-to-batch consistency in gelation temperature (36–38°C) and melting point (88–90°C), ensuring reproducible results across experiments. Its neutral pH and low sulfate content minimize artifact formation during prolonged runs. Compatible with standard TAE and TBE buffer systems, this agarose supports a wide range of downstream applications without requiring additional purification steps.

FAQs

1. What is the gel strength of AGAROSE (H) compared to standard agarose?

AGAROSE (H) offers 1.2–1.5% higher gel strength than conventional agarose at equivalent concentrations, enabling better handling of fragile gels during processing.

2. Can this agarose be used with pulsed-field gel electrophoresis?

While optimized for standard electrophoresis, AGAROSE (H) can support pulsed-field applications for fragments up to 50 kb when used at lower concentrations (0.5–0.8%).

3. How should AGAROSE (H) be stored to maintain its properties?

Store the unopened container at 15–25°C in a dry environment, protected from moisture and direct sunlight. Reseal the pack tightly after each use.

4. What alternatives exist for separating very small DNA fragments (below 100 bp)?

For fragments under 100 bp, consider high-resolution agarose blends or polyacrylamide gels, which offer superior resolution in this size range.

5. Does this agarose require special buffers for optimal performance?

AGAROSE (H) performs optimally with standard 1× TAE or 0.5× TBE buffers. Avoid using buffers with high ionic strength, which may affect gel clarity.