Description

Specifications Table

Product Name – AGAROSE (LOW MELTING) DNA grade
Quantity/Pack Size – 5 g, 10 g, 100 g
Form – Powder
Grade – DNA Grade
Application – Gel Electrophoresis

Product Overview

Low melting agarose DNA grade is a highly refined polysaccharide designed for superior performance in nucleic acid gel electrophoresis. Its unique low melting and gelling temperature (65°C and 24-28°C, respectively) allows for easy recovery of intact DNA fragments after separation, making it indispensable for applications requiring downstream processing like cloning or sequencing. This grade exhibits exceptional clarity, minimal background fluorescence, and ultra-low electroendosmosis (EEO), ensuring crisp band resolution even at high voltages. The product is rigorously tested to be free from nucleases (DNase/RNase) and other contaminants that could degrade sensitive samples. Its consistent lot-to-lot performance guarantees reproducible results across experiments. The powder dissolves rapidly in standard buffers, forming homogeneous gels with uniform pore sizes optimized for separating DNA fragments ranging from 50 bp to 20 kb. Whether used for analytical or preparative purposes, this agarose delivers unmatched purity and reliability for molecular biology workflows.

FAQs

1. What is the melting and gelling temperature of this low melting agarose?

The melting temperature is approximately 65°C, while the gelling temperature ranges between 24-28°C, allowing for easy handling during gel preparation and DNA recovery.

2. Can this agarose be used for pulse-field gel electrophoresis?

While optimized for standard electrophoresis, its low EEO and high purity make it suitable for certain PFGE applications, though specialized agarose may be preferred for large DNA fragments.

3. How should this agarose be stored to maintain its quality?

Store the unopened container at room temperature (15-30°C) in a dry environment, away from direct sunlight and moisture to prevent degradation.

4. What alternatives exist for high-resolution DNA separation?

For fragments below 50 bp, consider using high-resolution agarose or polyacrylamide gels, though these may require different handling protocols.

5. Is this agarose compatible with common DNA stains like ethidium bromide?

Yes, it is fully compatible with ethidium bromide and other nucleic acid stains, providing clear visualization under UV light without interfering with staining efficiency.