Description
Specifications Table
| Product Name | N,O-BIS-(TRIMETHYLSILYL) TRIFLUORO ACETAMIDE 98% |
|---|---|
| Quantity/Pack Size | 25 ml, 100 ml |
| Form | Liquid |
| Grade | Analytical Grade |
| Application | GC-MS derivatization, silylation reagent |
Product Description
N,O-BIS-(TRIMETHYLSILYL) TRIFLUORO ACETAMIDE, commonly known as BSTFA, is a high-purity derivatization reagent widely used in gas chromatography-mass spectrometry (GC-MS). This silylation reagent enhances the volatility and thermal stability of polar compounds, making it indispensable for analytical chemists and researchers.
With a purity of 98%, this BSTFA reagent ensures reliable and reproducible results in derivatization processes. Whether you’re analyzing metabolites, drugs, or environmental samples, this reagent simplifies complex mixtures for accurate GC-MS analysis.
At eqipped, we provide lab-grade BSTFA reagent in convenient pack sizes of 25 ml and 100 ml, catering to both small-scale research and bulk requirements. Our product is sourced from Loba Chemie, a trusted name in laboratory chemicals, ensuring consistency and performance.
Upgrade your GC-MS workflow with high-quality BSTFA reagent. Shop now at eqipped for fast delivery across India.
How BSTFA Reagent Works in Derivatization
BSTFA reacts with active hydrogen atoms in compounds like alcohols, amines, and carboxylic acids, replacing them with trimethylsilyl (TMS) groups. This modification reduces polarity, improving chromatographic separation and detection sensitivity.
Key advantages of using BSTFA include:
- Enhanced volatility for better GC separation
- Improved peak shape and resolution
- Compatibility with a wide range of functional groups
- Fast reaction times under mild conditions
Applications of BSTFA Reagent
This versatile silylation reagent is essential in various fields:
- Pharmaceutical Analysis: Derivatization of drugs and metabolites for pharmacokinetic studies.
- Environmental Testing: Analysis of pesticides, herbicides, and pollutants in soil/water samples.
- Food Safety: Detection of contaminants, additives, and residues in food products.
- Forensic Science: Identification of illicit substances and toxicology screening.
Safety and Handling
Risk Note: BSTFA is a moisture-sensitive and corrosive chemical. Handle with care in a fume hood, wearing appropriate PPE (gloves, goggles, lab coat). Store in a cool, dry place away from incompatible substances.
FAQs
What is BSTFA reagent used for?
BSTFA is primarily used as a derivatization reagent in GC-MS to convert polar compounds into volatile, stable derivatives for better analysis.
How do I prepare samples with BSTFA?
Dissolve your sample in an appropriate solvent (e.g., acetonitrile), add BSTFA (typically 1:1 ratio), and heat at 60–80°C for 15–30 minutes.
Is BSTFA compatible with all GC columns?
Yes, BSTFA derivatives are compatible with standard non-polar and mid-polar GC columns like 5% phenyl methylpolysiloxane.
What is the shelf life of BSTFA reagent?
When stored properly in a tightly sealed container, BSTFA has a shelf life of 2–3 years. Avoid exposure to moisture.
Can I use BSTFA for LC-MS?
No, BSTFA is specifically designed for GC-MS. For LC-MS, consider alternative derivatization agents like dansyl chloride.
