Description
Specifications Table
Product Name – BIS-TRIS 99% Molecular Biology
Quantity/Pack Size – 25 g, 100 g
Form – Powder
Grade – Molecular Biology (99%)
Application – Buffering agent for protein/DNA/RNA research
Product Overview
BIS-TRIS (2,2-Bis(hydroxymethyl)-2,2′,2”-nitrilotriethanol) is a high-purity zwitterionic buffer widely used in molecular biology for its superior buffering range (pH 5.8–7.2). This 99% grade ensures minimal impurities, making it ideal for sensitive applications like gel electrophoresis, protein crystallization, and nucleic acid hybridization. Its low UV absorbance and high solubility in water enhance compatibility with downstream assays. Unlike traditional buffers, BIS-TRIS maintains stable pH even at low temperatures, ensuring reproducibility in critical experiments. The powder form allows for flexible concentration adjustments, while its non-toxic and non-ionic nature minimizes interference with biological samples. Whether used in Tris-Bis-Tris gels or as a component in enzyme reaction buffers, this reagent delivers consistent performance for demanding research protocols.
FAQs
1. What is the pH range of BIS-TRIS buffer?
BIS-TRIS has an effective buffering range of pH 5.8 to 7.2, making it suitable for physiological and near-neutral applications.
2. Can BIS-TRIS replace Tris buffer in SDS-PAGE?
Yes, BIS-TRIS is often preferred over Tris in Bis-Tris gels for SDS-PAGE due to its sharper protein band resolution and compatibility with low-pH conditions.
3. Is this product compatible with mass spectrometry?
Yes, the 99% purity and low salt content minimize ion suppression, making it suitable for LC-MS and MALDI-TOF applications.
4. How should BIS-TRIS powder be stored?
Store in a cool, dry place at room temperature (15–25°C) in a tightly sealed container to prevent moisture absorption.
5. What alternatives exist for BIS-TRIS in protein work?
Alternatives include HEPES (pH 6.8–8.2) or MES (pH 5.5–6.7), but BIS-TRIS offers better stability for Bis-Tris gel systems.
