Description
Specifications Table
Product Name – CHAPSO 96% For Biochemistry
Quantity/Pack Size – 1 g, 5 g
Form – Powder
Grade – Biochemistry Grade
Application – Protein solubilization, membrane research
Product Overview
CHAPSO 96% is a high-purity zwitterionic detergent widely used in biochemistry for its exceptional ability to solubilize membrane proteins without denaturation. This non-ionic detergent maintains protein structure and activity, making it indispensable for studies involving membrane-associated proteins, enzyme assays, and structural biology. Its mild nature ensures minimal disruption to native protein conformations, while its high purity (96%) guarantees reliable and reproducible results in sensitive applications. The powder form allows for precise dosing and easy integration into various buffer systems. Whether used in protein purification, crystallization, or functional assays, CHAPSO 96% delivers consistent performance, reducing background interference and improving downstream analysis. Its compatibility with a wide range of biochemical techniques makes it a versatile choice for researchers demanding high-quality reagents for critical experiments.
FAQs
1.
What is the chemical structure of CHAPSO?
CHAPSO, or 3-[(3-cholamidopropyl)dimethylammonio]-2-hydroxy-1-propanesulfonate, is a zwitterionic detergent derived from cholate with a sulfonate head group and a hydroxyl group. 2.
Is CHAPSO compatible with mass spectrometry?
Yes, CHAPSO is compatible with mass spectrometry due to its low UV absorbance and minimal interference with ionization processes, though it should be removed or diluted for optimal results. 3.
How does CHAPSO compare to CHAPS?
CHAPSO is structurally similar to CHAPS but contains an additional hydroxyl group, which can influence its solubility properties and mildness in certain applications. 4.
Can CHAPSO be used for cell lysis?
While CHAPSO is primarily used for protein solubilization, it can be included in lysis buffers for gentle cell membrane disruption, especially in combination with other detergents. 5.
What is the recommended storage condition for CHAPSO?
Store CHAPSO in a cool, dry place away from direct sunlight, ideally at room temperature in a tightly sealed container to prevent moisture absorption.
