Specifications Table

Product Name – KOVAC’S INDOLE REAGENT
Quantity/Pack Size – 100 ml, 500 ml
Form – Liquid
Grade – Laboratory Reagent
Application – Indole detection in bacterial cultures

Product Overview

Kovac?s Indole Reagent is a specialized chemical solution designed for the rapid and accurate detection of indole production in bacterial cultures. This reagent is widely used in microbiology laboratories to differentiate bacterial species based on their ability to metabolize tryptophan into indole. The reagent contains paradimethylaminobenzaldehyde (DMAB) dissolved in a mixture of amyl or isoamyl alcohol and hydrochloric acid, which reacts with indole to form a red or pink coloration in the presence of a positive result. This color change provides a clear visual confirmation, making it an essential tool for bacterial identification. The reagent is formulated to ensure stability and consistency, delivering reliable results with minimal interference. It is compatible with standard microbiological protocols and can be used directly without additional preparation. The high-purity grade ensures accuracy, making it suitable for both routine and research applications. Proper storage in a cool, dark place extends its shelf life, maintaining its efficacy over time.

FAQs

1. How does Kovac?s Indole Reagent work in bacterial testing?

The reagent reacts with indole, a metabolic byproduct of tryptophan breakdown in certain bacteria, producing a red or pink color upon contact, indicating a positive result.

2. Is this reagent compatible with automated bacterial identification systems?

Yes, Kovac?s Indole Reagent can be integrated into automated systems, provided the protocol accounts for manual reagent addition where required.

3. What are the storage conditions for Kovac?s Indole Reagent?

Store the reagent in a tightly sealed container at room temperature, away from direct sunlight and moisture, to maintain stability and effectiveness.

4. Can this reagent be used for environmental bacterial testing?

While primarily designed for clinical and lab-based bacterial identification, it can also be applied to environmental samples if the bacteria produce indole.

5. Are there any safety precautions when handling this reagent?

Wear appropriate protective gear, including gloves and goggles, as the reagent contains alcohol and acid, which may cause irritation upon contact with skin or eyes.