Description
Specifications Table
Product Name – 4-NITROPHENYL PHOSPHATE 96% Extra Pure
Quantity/Pack Size – 10 g, 50 g
Form – Solid
Grade – Extra Pure
Application – Enzymatic assays, biochemical research
Product Overview
4-Nitrophenyl Phosphate 96% Extra Pure is a high-grade biochemical reagent widely used in enzymatic assays and research applications. This compound serves as a substrate for alkaline phosphatase, producing a yellow-colored product (4-nitrophenol) upon hydrolysis, which can be quantified spectrophotometrically at 405 nm. The extra pure grade ensures minimal impurities, providing consistent and reliable results in sensitive experiments. Its stability under standard laboratory conditions makes it ideal for long-term storage without degradation. The solid form allows for easy handling and precise weighing, ensuring accuracy in experimental setups. Whether used in qualitative or quantitative assays, this reagent delivers high reproducibility, making it a preferred choice for researchers and students alike. The compound?s high purity level minimizes background interference, enhancing the sensitivity of detection methods. Compatible with most aqueous buffers, it integrates seamlessly into existing protocols without requiring extensive modifications.
FAQs
1. What is the solubility of 4-Nitrophenyl Phosphate in water?
4-Nitrophenyl Phosphate is sparingly soluble in water but dissolves well in alkaline solutions and organic solvents like DMSO.
2. Can this compound be used for colorimetric assays?
Yes, it is commonly used in colorimetric assays due to the yellow product formed upon enzymatic hydrolysis.
3. What is the shelf life of this reagent?
When stored in a cool, dry place, the reagent remains stable for at least 2 years from the date of manufacture.
4. Is this product compatible with standard lab buffers?
Yes, it is compatible with most aqueous buffers used in biochemical assays.
5. What precautions should be taken while handling this chemical?
Wear appropriate protective gear, including gloves and goggles, and handle in a well-ventilated area to avoid inhalation.
