Description
Specifications Table
Product Name – Bicine, 99+%, for analysis
Quantity/Pack Size – 1KG, 100GR, 250GR
Form – Powder
Grade – 99%+
Application – Biochemical buffer, pH stabilization
Product Overview
Bicine (N,N-bis(2-hydroxyethyl)glycine) is a high-purity zwitterionic buffer with exceptional pH stability, making it indispensable for biochemical and molecular biology applications. With a purity of 99%+, this analytical-grade compound ensures minimal interference in sensitive assays, electrophoresis, and protein studies. Its low toxicity and excellent solubility in water (up to 1M) allow for precise buffer preparation without compromising cellular or enzymatic activity. The buffer’s effective pH range (7.6–9.0) aligns perfectly with physiological conditions, reducing the need for pH adjustments during experiments. Unlike traditional buffers like Tris or HEPES, Bicine exhibits minimal temperature-dependent pH shifts, ensuring reproducibility across varying lab conditions. Its chemical stability under autoclaving (121°C) further enhances its utility in sterile applications. Whether used in protein purification, DNA/RNA hybridization, or cell culture media, this Bicine formulation guarantees consistent performance, reducing batch-to-batch variability. The powder form allows for flexible concentration adjustments, while its non-volatile nature prevents loss during prolonged incubations. For researchers demanding reliability in pH-critical workflows, this 99%+ pure Bicine delivers unmatched precision and compatibility with downstream analytical techniques.
FAQs
1. What is the effective pH range for Bicine buffer solutions?
Bicine functions optimally between pH 7.6 and 9.0, with a pKa of 8.35 at 20°C, making it suitable for near-physiological applications where minimal pH drift is critical.
2. Can Bicine be autoclaved without degradation?
Yes, Bicine solutions (up to 0.1M) remain stable when autoclaved at 121°C for 20 minutes, though higher concentrations may require filter sterilization to prevent precipitation.
3. How does Bicine compare to HEPES for cell culture applications?
Bicine offers a broader alkaline pH range (7.6–9.0) compared to HEPES (6.8–8.2), but HEPES has lower membrane permeability, which may be preferable for long-term cell culture stability.
4. What is the shelf life of unopened Bicine powder?
When stored desiccated at room temperature (15–25°C) in its original packaging, unopened Bicine retains ≥99% purity for up to 3 years from the date of manufacture.
5. Are there any known incompatibilities with metal ions?
Bicine forms complexes with divalent cations like Cu²⁺, Zn²⁺, and Fe³⁺, which may interfere with metalloenzyme assays; EDTA or alternative buffers are recommended for such applications.
