Buffer solution pH 7, Phosphate buffer | 258590010 | Thermofisher

Description

Specifications Table

Product Name – Buffer solution pH 7, Phosphate buffer
Quantity/Pack Size – 100ML, 500ML, 1LT, 2.5LT
Form – Liquid
Grade – Laboratory Grade
Application – Biochemical assays, cell culture, analytical testing

Product Overview

This pH 7 phosphate buffer solution is a meticulously formulated laboratory reagent designed to provide consistent pH stability for a wide range of biochemical and analytical applications. Composed of monobasic and dibasic sodium phosphate salts, this buffer maintains a precise pH of 7.0, making it ideal for experiments requiring neutral conditions. The laboratory-grade purity ensures minimal interference with sensitive assays, while its balanced ionic strength supports optimal enzyme activity and protein stability. The solution is pre-sterilized and ready-to-use, eliminating the need for time-consuming preparation while guaranteeing reproducibility across experiments. Its stable formulation prevents pH drift over extended periods, even during temperature fluctuations common in lab environments. The phosphate-based system offers excellent buffering capacity in the physiological pH range, making it particularly valuable for cell culture applications where maintaining osmotic balance is critical. Each batch undergoes rigorous quality control testing to verify pH accuracy and absence of contaminants, ensuring reliable performance in critical research applications.

FAQs

1. What is the exact pH range this buffer maintains effectively?

This phosphate buffer solution is most effective in maintaining pH between 6.8 and 7.4, with optimal performance at the specified pH 7.0.

2. Can this buffer be autoclaved for sterile applications?

The solution is pre-sterilized and doesn’t require autoclaving, but it can withstand standard autoclaving procedures at 121°C for 15 minutes if additional sterilization is needed.

3. What materials are incompatible with phosphate buffers?

Phosphate buffers may form insoluble precipitates with calcium, magnesium, and other divalent cations at higher concentrations.

4. How should this buffer be stored for long-term stability?

Store the unopened container at room temperature (15-25°C) away from direct sunlight. Once opened, store at 4°C and use within 6 months for optimal performance.

5. What alternatives exist for applications requiring different pH ranges?

For acidic applications, consider acetate buffers (pH 3.6-5.6) or citrate buffers (pH 3.0-6.2), while Tris or HEPES buffers work well for alkaline conditions.