Description
Specifications Table
Product Name – CHES, 98+%, for biochemistry
Quantity/Pack Size – 25GR, 100GR, 500GR
Form – Powder
Grade – Biochemistry Grade
Application – Buffering agent in biochemical assays
Product Overview
CHES (2-(N-Cyclohexylamino)ethanesulfonic acid) is a high-purity zwitterionic buffer with a pKa of 9.3, making it ideal for maintaining stable pH in biochemical applications. This 98+% grade ensures minimal interference in sensitive assays, offering consistent performance in protein studies, enzyme reactions, and nucleic acid research. Its excellent water solubility and low metal ion content enhance reliability in critical experiments. The powder form allows for flexible preparation of solutions at varying concentrations, catering to diverse laboratory requirements. As a non-toxic, non-volatile buffer, CHES provides a safer alternative to traditional buffering systems while maintaining high efficiency. The product undergoes rigorous quality control to meet international biochemistry standards, ensuring reproducibility in research protocols. Its chemical stability across a wide temperature range further extends its utility in long-duration experiments. The absence of UV absorbance in the 230-300nm range makes it particularly suitable for spectrophotometric applications.
FAQs
1. What is the effective pH range for CHES buffer?
The optimal buffering range for CHES is between pH 8.6 and 10.0, with maximum capacity at pH 9.3.
2. How should CHES buffer be stored for long-term use?
Store the powder in a tightly sealed container at room temperature, protected from moisture and direct sunlight.
3. Is this CHES buffer compatible with common laboratory detergents?
Yes, it maintains stability when used with mild non-ionic detergents like Triton X-100 in most applications.
4. What alternatives exist for applications requiring lower pH buffers?
For pH ranges below 8.6, consider HEPES (pKa 7.5) or TAPS (pKa 8.4) as suitable alternatives.
5. Does this CHES formulation contain any animal-derived components?
No, this is a synthetic product with no animal-derived ingredients in its composition.
