Description
Specifications Table
Product Name – HEPES, 99%, for biochemistry
Quantity/Pack Size – 25GR, 100GR, 250GR, 1KG, 5KG
Form – White crystalline powder
Grade – Biochemistry grade, 99% purity
Application – pH buffering in cell culture, protein purification, molecular biology
Product Overview
HEPES (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid) is a zwitterionic organic buffering agent widely recognized for its superior performance in biological systems. This 99% pure biochemistry-grade HEPES offers minimal toxicity and exceptional stability across a broad pH range (6.8–8.2), making it the preferred choice for sensitive applications where pH fluctuations must be tightly controlled. Unlike bicarbonate buffers, HEPES maintains consistent pH levels even in open systems, eliminating the need for CO₂ equilibration. Its high solubility in water (up to 1.5 M) and compatibility with most cell culture media components ensure seamless integration into existing protocols. The product undergoes rigorous quality control to guarantee freedom from heavy metal contaminants, endotoxins, and DNase/RNase activity, preserving the integrity of experimental results. Its low temperature coefficient (ΔpKa/°C = -0.014) ensures reliable performance across varying incubation conditions, while the crystalline powder form allows for precise weighing and long-term storage stability. Whether used in mammalian cell culture, protein crystallization, or enzymatic assays, this HEPES buffer delivers consistent osmotic balance and cellular viability, reducing experimental variability.
FAQs
1. What is the optimal working concentration for HEPES in cell culture media?
Typical concentrations range from 10–25 mM, though this may vary depending on the cell type and specific application. For most mammalian cell lines, 20 mM provides effective buffering without osmotic stress.
2. Can this HEPES be used for DNA/RNA extraction protocols?
Yes, the 99% purity and absence of nucleases make it suitable for nucleic acid extraction buffers, though it’s primarily formulated for cell culture and protein-based applications.
3. How does HEPES compare to phosphate buffers for protein studies?
HEPES offers a wider effective pH range (6.8–8.2) and doesn’t precipitate calcium or magnesium ions, unlike phosphate buffers, making it ideal for enzymatic assays involving metal cofactors.
4. What’s the recommended storage condition to maintain stability?
Store the unopened powder at room temperature (15–25°C) in a dry environment. After opening, seal tightly to prevent moisture absorption, which can alter the buffering capacity.
5. Is this HEPES compatible with serum-free media formulations?
The biochemistry-grade purity ensures compatibility with serum-free and chemically defined media, though we recommend validating performance with your specific cell line.
