Description
Specifications Table
Product Name – MES monohydrate, 99+%, for biochemistry
Quantity/Pack Size – 100GR, 500GR, 2.5KG
Form – White crystalline powder
Grade – Biochemistry grade, 99+% purity
Application – Buffering agent in biochemical assays, cell culture, protein purification
Product Overview
MES monohydrate (2-(N-morpholino)ethanesulfonic acid monohydrate) is a high-purity biochemical buffer with a molecular weight of 213.26 g/mol and a pH buffering range of 5.5–6.7. This 99+% pure grade is specifically formulated for applications requiring precise pH control, such as protein crystallization, enzyme assays, and cell culture media preparation. Unlike traditional buffers, MES exhibits minimal metal ion chelation and low toxicity, making it suitable for sensitive biological systems. Its zwitterionic nature ensures stability across a broad temperature range, while the monohydrate form guarantees consistent solubility in aqueous solutions. The compound’s resistance to enzymatic degradation and lack of absorbance in the UV-Vis spectrum further enhance its utility in spectrophotometric analyses. Each batch undergoes rigorous quality testing to confirm purity, pH performance, and absence of endotoxins or heavy metal contaminants. The fine crystalline structure allows for rapid dissolution without residue, ensuring reproducibility in experimental protocols.
FAQs
1. What is the ideal pH range for MES monohydrate as a buffer?
MES monohydrate functions optimally between pH 5.5 and 6.7, making it ideal for slightly acidic biochemical applications where phosphate buffers may interfere.
2. Can this MES grade be used for cell culture media?
Yes, this 99+% pure MES monohydrate is compatible with cell culture systems due to its low cytotoxicity and absence of growth-inhibiting impurities.
3. How does MES compare to HEPES buffer in protein studies?
MES is preferred for applications requiring lower pH (5.5–6.7), while HEPES covers 6.8–8.2. MES also has minimal metal ion binding, reducing interference in metalloenzyme assays.
4. What is the recommended storage condition for this product?
Store in a tightly sealed container at room temperature (15–25°C), protected from moisture and direct light to prevent hydration changes or degradation.
5. Is this MES monohydrate suitable for HPLC mobile phase preparation?
Yes, its high purity and UV transparency make it suitable for HPLC applications, though it’s primarily used as a buffer rather than a solvent modifier.
