Specifications Table

Product Name – 1-(DIMETHYL AMINOPROPYL)-3-ETHYL CARBODIIMIDE HYDROCHLORIDE
Quantity/Pack Size – 5 g, 25 g, 100 g, 500 g
Form – White to off-white crystalline powder
Grade – Extra Pure (98%)
Application – Peptide synthesis, protein cross-linking, amide bond formation

Product Overview

1-(Dimethylaminopropyl)-3-ethyl carbodiimide hydrochloride (EDC HCl) is a high-purity, water-soluble carbodiimide reagent used extensively in biochemical coupling reactions. This extra pure (98%) grade ensures minimal impurities, making it ideal for sensitive applications requiring precise amide bond formation. The compound functions as a zero-length crosslinker, facilitating direct conjugation between carboxyl and amino groups without becoming part of the final product. Its crystalline powder form offers excellent stability under standard laboratory conditions. EDC HCl is particularly valued for its efficiency in activating carboxyl groups for subsequent reaction with amines, producing stable amide bonds. The reagent’s solubility in water allows for easy preparation of reaction mixtures, while its high purity minimizes side reactions that could compromise experimental results. Proper storage in a cool, dry environment preserves its reactivity over extended periods. This grade is specifically processed to meet stringent quality standards for research applications where consistency and reliability are critical.

FAQs

1. What is the shelf life of EDC HCl when stored properly?

When stored in a tightly sealed container at 2-8°C, protected from moisture and light, EDC HCl maintains its specified purity for at least 24 months from the date of manufacture. Always check the container for signs of moisture absorption before use.

2. Can this grade of EDC HCl be used for large-scale synthesis?

While primarily designed for laboratory-scale applications, this extra pure grade can be used for pilot-scale synthesis. For industrial-scale production, specialized bulk grades with different handling characteristics may be more appropriate.

3. What safety precautions should be taken when handling EDC HCl?

Always wear appropriate personal protective equipment including gloves, safety goggles, and a lab coat. Work in a well-ventilated fume hood as EDC HCl can release irritating vapors. Avoid inhalation and direct contact with skin or eyes.

4. How does the purity of this EDC HCl compare to standard grades?

This extra pure 98% grade contains significantly fewer impurities than standard technical grades (typically 95-97% pure). The reduced impurity profile minimizes side reactions and improves yield consistency in sensitive applications.

5. What is the typical reaction time when using EDC HCl for coupling?

Reaction times typically range from 2-24 hours depending on the specific application, concentration, and reaction conditions. Most peptide coupling reactions reach completion within 4-12 hours at room temperature with proper catalysis.