Description
Specifications Table
Product Name – Heptanesulfonic acid sodium salt, anhydrous HPLC
Quantity/Pack Size – 25 g
Form – Solid (powder/crystals)
Grade – HPLC (High-Performance Liquid Chromatography)
Application – Ion-pair reagent for reverse-phase HPLC, mobile phase modifier
Product Overview
Heptanesulfonic acid sodium salt, anhydrous, is a high-purity ion-pairing reagent specifically designed for HPLC applications where precise control over retention times and peak symmetry is critical. This HPLC-grade salt acts as a hydrophobic counterion, enhancing the separation of polar and ionic compounds in reverse-phase chromatography. Its anhydrous form eliminates moisture-related variability, ensuring consistent performance across analytical runs. The compound’s sulfonic acid group provides strong ion-pairing interactions, making it ideal for separating amines, amino acids, and other charged analytes. With minimal UV absorbance at common detection wavelengths, it maintains baseline stability without interfering with analyte detection. The product undergoes rigorous purification to remove trace metals and organic impurities, meeting the stringent requirements of pharmaceutical, environmental, and biochemical analysis. Its compatibility with aqueous and organic mobile phases allows flexible method development, while the sodium salt form ensures solubility and stability in typical HPLC buffers. Whether used for gradient or isocratic separations, this reagent delivers reproducible results, reducing the need for frequent column re-equilibration or system recalibration.
FAQs
1. What is the role of heptanesulfonic acid sodium salt in HPLC?
It functions as an ion-pairing reagent, neutralizing charged analytes to improve their retention and separation on reverse-phase columns by forming neutral ion pairs.
2. Can this product be used with mass spectrometry (LC-MS) detection?
While effective for UV/Vis detection, its non-volatile nature may cause ion suppression in LC-MS; volatile alternatives like trifluoroacetic acid are often preferred for MS compatibility.
3. How should this reagent be stored to maintain stability?
Store in a tightly sealed container at room temperature (15–25°C), protected from moisture and light to prevent hydrolysis or degradation of the anhydrous form.
4. What pH range is optimal for using this ion-pair reagent?
The reagent performs best in acidic to neutral pH ranges (2.5–7.0), where the sulfonic acid group remains ionized for effective ion-pair formation.
5. Are there alternatives for separating highly polar compounds?
For extremely polar analytes, shorter-chain ion-pair reagents (e.g., pentanesulfonic acid) or HILIC (Hydrophilic Interaction Liquid Chromatography) may be explored as alternatives.
