Specifications Table

Product Name – 1-Hexanesulfonic acid, sodium salt hydrate
Quantity/Pack Size – 25GR, 5GR, 100GR, 500GR
Form – Solid (hydrated crystals)
Grade – HPLC (98% purity)
Application – Ion-pair reagent for reverse-phase HPLC, LC-MS compatibility

Product Overview

1-Hexanesulfonic acid sodium salt hydrate is a high-purity (98%) ion-pairing reagent specifically formulated for HPLC and LC-MS applications. Its hydrated crystalline form ensures consistent solubility in aqueous mobile phases, while the sodium salt structure minimizes UV absorbance interference, making it ideal for sensitive chromatographic separations. The reagent’s long hydrophobic chain (C6) provides effective retention modulation for polar analytes, particularly in reverse-phase chromatography systems. Its low volatility and stability under standard HPLC conditions reduce baseline drift, improving peak resolution and reproducibility. The hydrate form maintains batch-to-batch consistency, critical for quantitative analysis. Compatible with most common HPLC columns, this reagent is pre-tested for minimal impurities, ensuring reliable performance in gradient elution protocols. The 98% purity grade meets stringent requirements for pharmaceutical, environmental, and biochemical analysis, where trace contaminants could compromise results. Store under desiccated conditions to preserve its hydrated state and prevent degradation from moisture fluctuations.

FAQs

1. What is the shelf life of this HPLC-grade sodium hexanesulfonate?

When stored in a tightly sealed container at room temperature (15-25°C) away from direct sunlight, the product retains its specified purity for 24 months from the date of manufacture. Avoid exposure to humidity to prevent clumping.

2. Can this reagent be used with mass spectrometry (LC-MS)?

Yes, the sodium salt form is compatible with LC-MS systems, though sodium adduct formation may occur. For sensitive MS detection, consider using the ammonium salt alternative or optimize source parameters to minimize sodium interference.

3. How does this compare to heptanesulfonic acid for ion-pairing?

1-Hexanesulfonic acid offers a shorter alkyl chain (C6 vs. C7), resulting in weaker retention for highly polar compounds but better elution of moderately hydrophobic analytes. It’s often preferred for separating small organic acids or bases where heptanesulfonate may over-retain.

4. What mobile phase pH range is optimal for this reagent?

The reagent performs best in acidic to neutral pH ranges (2.5–7.0). Below pH 2.5, protonation of the sulfonate group may reduce ion-pairing efficiency, while above pH 7.0, precipitation risks increase, especially with divalent cations.

5. Is this product suitable for preparative HPLC?

While primarily designed for analytical HPLC, it can be used in preparative scales if the column and system are compatible with sodium salts. Ensure thorough post-run cleaning to prevent salt buildup in the system.