Description
Specifications Table
Product Name – 1-Octanesulfonic acid, sodium salt, HPLC grade
Quantity/Pack Size – 5GR, 25GR, 100GR, 500GR
Form – White to off-white crystalline powder
Grade – HPLC (≥99% purity)
Application – Ion-pair reagent for reverse-phase HPLC, LC-MS, and chromatographic separations of polar compounds
Product Overview
1-Octanesulfonic acid sodium salt is a high-purity ion-pair reagent specifically designed for reverse-phase high-performance liquid chromatography (HPLC) and liquid chromatography-mass spectrometry (LC-MS) applications. This HPLC-grade compound ensures minimal UV absorbance at wavelengths above 210 nm, making it ideal for sensitive detection methods without interfering with analyte signals. Its hydrophobic alkyl chain (C8) and sulfonic acid group provide optimal interaction with both stationary and mobile phases, enhancing retention and separation of polar or ionic analytes such as amines, organic acids, and nucleotides. The sodium salt form improves solubility in aqueous and polar organic solvents, including methanol and acetonitrile, ensuring consistent performance across gradient elution protocols. The product undergoes rigorous quality control to guarantee lot-to-lot reproducibility, with residual impurities (e.g., heavy metals, inorganic salts) maintained below 0.1%. Its low volatility and thermal stability make it suitable for prolonged storage under ambient conditions, though sealing the container tightly after use is recommended to prevent moisture absorption. Compatible with standard HPLC columns (C18, C8) and mobile phases, this reagent eliminates the need for complex buffer preparations, simplifying method development. For researchers requiring ultra-low background noise, its high purity reduces baseline drift and ghost peaks, critical for quantitative analysis in pharmaceutical, environmental, and biochemical laboratories. The powder form allows flexible dosing, from micro-scale analytical runs to preparative chromatography.
FAQs
1. What is the primary role of 1-octanesulfonic acid sodium salt in HPLC?
It acts as an ion-pair reagent by forming neutral complexes with charged analytes, improving their retention and separation on reverse-phase columns without requiring extreme pH adjustments.
2. Can this reagent be used with LC-MS systems?
Yes, its high purity and low UV absorbance make it compatible with LC-MS, though volatile alternatives like trifluoroacetic acid may be preferred for certain ionization techniques to avoid salt buildup.
3. How does the C8 chain length compare to shorter or longer alkyl sulfonates?
The C8 chain offers a balanced hydrophobicity—stronger retention than C4-C6 analogs but less risk of column fouling compared to C12-C18 reagents, making it versatile for mid-polarity compounds.
4. What solvents are recommended for preparing the mobile phase?
It dissolves readily in water, methanol, and acetonitrile. For gradient elution, pre-dissolve in the aqueous component (e.g., 5–20 mM in water) before mixing with organic modifiers to prevent precipitation.
5. Are there storage precautions to maintain stability?
Store in a cool, dry place with the container tightly sealed. While stable at room temperature, prolonged exposure to humidity may cause clumping; desiccants can be added to the storage bottle if needed.
