Description

Specifications Table

Product Name – 1-Pentanesulfonic acid sodium salt, anhydrous
Quantity/Pack Size – 25 g
Form – Solid (powder/crystals)
Grade – HPLC (≥99.0% purity)
Application – Ion-pair reagent for reverse-phase HPLC, LC-MS

Product Overview

This anhydrous 1-pentanesulfonic acid sodium salt is a high-purity ion-pairing reagent specifically formulated for HPLC and LC-MS applications. Its ≥99.0% purity ensures minimal interference with chromatographic separations, while the anhydrous form eliminates moisture-related variability. The compound’s hydrophobic alkyl chain (C5) and sulfonic acid group create strong ion-pair interactions with basic analytes, improving peak symmetry and retention reproducibility. Unlike shorter-chain analogs, this reagent provides optimal selectivity for mid-polarity compounds without excessive column fouling. Its low UV absorbance at common detection wavelengths (210–254 nm) prevents baseline drift, while the sodium salt form enhances solubility in aqueous mobile phases. The crystalline structure resists hygroscopicity, maintaining stability during storage and handling. Compatible with standard C18 and phenyl-hexyl stationary phases, this reagent is ideal for methods requiring gradient elution or isocratic conditions. Each batch undergoes rigorous testing for residual metals, organic impurities, and pH consistency to meet HPLC-grade specifications.

FAQs

1. What is the solubility of this reagent in methanol or acetonitrile?

Fully soluble in methanol (≥50 mg/mL) and acetonitrile (≥30 mg/mL) at room temperature. Pre-warming the solvent to 40°C can accelerate dissolution for concentrated stock solutions.

2. Can this be used with mass spectrometry (LC-MS) without ion suppression?

Yes, but limit concentrations to ≤5 mM in the mobile phase. Higher levels may cause signal suppression due to sodium adduct formation. Use ammonium salts as alternatives if sensitivity is critical.

3. How does this compare to hexanesulfonic acid sodium salt?

1-Pentanesulfonic acid offers shorter retention times for polar analytes and lower viscosity in mobile phases. Hexanesulfonic acid provides stronger hydrophobic interactions but may require longer re-equilibration times.

4. What’s the recommended storage condition to prevent degradation?

Store in a tightly sealed container at 2–8°C, protected from light and moisture. Avoid repeated freeze-thaw cycles, as condensation can introduce water into the anhydrous salt.

5. Is this compatible with phosphate or acetate buffers in the mobile phase?

Compatible, but precipitate formation may occur at pH 7.0. Test compatibility by mixing small volumes before preparing bulk mobile phases.