Specifications Table

Product Name – 1-Pentanesulfonic acid, sodium salt monohydrate
Quantity/Pack Size – 25GR, 100GR
Form – Solid (powder/crystals)
Grade – HPLC grade (98%+ purity)
Application – Ion-pair reagent, HPLC mobile phase modifier, analytical separations

Product Overview

1-Pentanesulfonic acid sodium salt monohydrate is a high-purity (98%+) ion-pairing reagent specifically designed for HPLC and analytical chromatography applications. This HPLC-grade compound ensures minimal interference in sensitive separations, making it ideal for complex sample matrices where precise retention control is critical. The sodium salt form enhances solubility in aqueous mobile phases, providing consistent performance across gradient and isocratic elution methods. Its monohydrate structure maintains stability during storage while preserving chromatographic integrity. The reagent’s low UV absorbance at common detection wavelengths (210-254 nm) minimizes baseline noise, improving signal-to-noise ratios in quantitative analyses. Compatible with reversed-phase and ion-exchange columns, this versatile modifier enables selective retention of ionic analytes, including peptides, nucleotides, and pharmaceutical compounds. The 25GR and 100GR pack sizes cater to both small-scale research and high-throughput laboratories, ensuring cost-effective usage without compromising quality. Rigorous quality control guarantees batch-to-batch consistency, with residual impurities (metals, organic volatiles) maintained below HPLC-grade thresholds. Store under inert conditions to prevent moisture absorption and degradation of sulfonic acid functionality.

FAQs

1. What is the primary function of 1-pentanesulfonic acid sodium salt in HPLC?

It acts as an ion-pairing reagent to modify the retention behavior of ionic analytes by forming neutral ion pairs, improving separation efficiency in reversed-phase chromatography.

2. Can this reagent be used with mass spectrometry (LC-MS) applications?

While effective for HPLC-UV, its non-volatile nature may require alternative ion-pairing agents or mobile phase additives for LC-MS to avoid ion source contamination and signal suppression.

3. How does the monohydrate form differ from anhydrous versions?

The monohydrate contains one molecule of water per formula unit, offering enhanced stability during storage but requiring consistent handling to prevent hydration-level variations that could affect reproducibility.

4. What precautions should be taken during mobile phase preparation?

Use HPLC-grade water and solvents to prepare mobile phases, and filter through 0.22 µm membranes to remove particulate matter. Adjust pH after adding the reagent to avoid precipitation.

5. Are there compatibility issues with stainless steel HPLC systems?

At neutral to acidic pH, it is generally compatible, but prolonged exposure to highly concentrated solutions (above 10 mM) may accelerate corrosion; rinse systems with water or organic solvents post-use.