Description
Specifications Table
| Product Name | 10X TAE Buffer |
|---|---|
| Pack Size | 2 × 500 ml |
| Form | Liquid |
| Grade | Molecular Biology |
| Application | Electrophoresis, DNA/RNA analysis |
Product Description
When it comes to reliable electrophoresis buffers, 10X TAE Buffer from Himedia stands out as a trusted choice for researchers, students, and educators. This high-quality buffer solution is specifically formulated to ensure optimal DNA and RNA separation during gel electrophoresis, making it an essential component in molecular biology labs.
TAE (Tris-Acetate-EDTA) buffer is widely preferred for its stability and ability to maintain a consistent pH during prolonged runs. The 10X concentration allows for easy dilution, providing flexibility in experimental setups. Whether you’re working on routine lab experiments or advanced research, this buffer delivers consistent results every time.
At eqipped, we understand the importance of precision in laboratory work. That’s why we source only the best supplies, ensuring that every product meets stringent quality standards. This 10X TAE Buffer is no exception—it’s manufactured by Himedia, a brand synonymous with reliability in lab reagents.
Need a dependable buffer for your next electrophoresis experiment? Buy 10X TAE Buffer online today and experience seamless performance in your lab work.
Why This Buffer Matters in Your Lab
Electrophoresis is a cornerstone technique in molecular biology, and the buffer you choose can significantly impact your results. TAE buffer is particularly valued for its sharp resolution of DNA fragments, especially in the 0.5–20 kb range. Unlike TBE buffer, TAE is gentler on DNA, making it ideal for applications where DNA recovery is necessary post-electrophoresis.
This 10X concentrate simplifies preparation—just dilute with distilled water to achieve the working 1X concentration. No more last-minute buffer preparation or worrying about inconsistent pH levels. With Himedia’s 10X TAE Buffer, you get a ready-to-use solution that saves time and reduces experimental variability.
Applications of 10X TAE Buffer
This versatile buffer is used in a variety of molecular biology applications, including:
- Agarose gel electrophoresis for DNA and RNA analysis
- PCR product verification
- Plasmid DNA separation
- Restriction enzyme digestion analysis
- Educational lab demonstrations
Whether you’re a student learning the basics of gel electrophoresis or a researcher analyzing complex genetic samples, this buffer provides the clarity and reliability you need.
How to Use 10X TAE Buffer
Using this buffer is straightforward. For a standard agarose gel, dilute the 10X solution to 1X with distilled water. For example, mix 100 ml of 10X TAE with 900 ml of water to prepare 1 liter of 1X TAE buffer. This diluted solution can be used both for gel preparation and as a running buffer.
Pro tip: Always use fresh buffer for critical experiments to avoid contamination or pH shifts that may affect your results.
Storage and Handling
Store the 10X TAE Buffer at room temperature (15–25°C) in a tightly sealed container. Avoid exposure to direct sunlight or extreme temperatures. When properly stored, this buffer remains stable for up to 12 months.
Looking for a hassle-free way to stock up on lab essentials? Shop now and get your 10X TAE Buffer delivered to your doorstep.
FAQs
What is the shelf life of 10X TAE Buffer?
The shelf life is 12 months when stored at room temperature in a sealed container. Always check for any signs of contamination before use.
Can I use TAE buffer for protein electrophoresis?
No, TAE buffer is specifically designed for nucleic acid (DNA/RNA) electrophoresis. For proteins, consider using Tris-Glycine or other protein-specific buffers.
How do I dispose of used TAE buffer?
Diluted TAE buffer can be disposed of as non-hazardous waste, but always follow your institution’s guidelines for chemical disposal.
Is this buffer compatible with ethidium bromide staining?
Yes, TAE buffer works well with ethidium bromide for DNA visualization under UV light.
Can I autoclave TAE buffer?
Autoclaving is not recommended as it may alter the buffer’s pH and effectiveness. Use sterile filtration if sterilization is required.
