Description
Specifications Table
| Product Name | 1,6-Diphenyl-1,3,5-Hexatriene (DPH) pure, 98% |
|---|---|
| Quantity/Pack Size | 1 Gms, 5 Gms |
| Form | Solid |
| Grade | Pure, 98% |
| Application | Fluorescence anisotropy studies, membrane fluidity research |
Product Description
1,6-Diphenyl-1,3,5-Hexatriene (DPH) is a highly sensitive fluorescent probe widely used in biochemistry and biophysics to study membrane fluidity and dynamics. With a purity of 98%, this DPH variant ensures accurate and reproducible results in fluorescence anisotropy experiments. Its rigid, rod-like structure makes it ideal for embedding in lipid bilayers, providing insights into membrane properties.
Researchers and educators rely on DPH for its stability and strong fluorescence emission when excited at the appropriate wavelength. Whether you’re investigating lipid phase transitions or cellular membrane behavior, this high-grade DPH from eqipped delivers consistent performance.
Need a reliable DPH chemical supplier? Order 1,6-Diphenyl-1,3,5-Hexatriene 98% online today for your lab experiments.
DPH is commonly dissolved in organic solvents like DMSO or ethanol before use. Its hydrophobic nature allows it to partition into lipid membranes, where its fluorescence properties change with the surrounding microenvironment. This makes it invaluable for studying membrane viscosity, phase transitions, and protein-lipid interactions.
For best results, store DPH in a cool, dry place away from direct light. Always handle with care, as it is sensitive to oxidation. eqipped ensures that each batch meets strict quality standards, so you can focus on your research without worrying about reagent variability.
FAQs
What is the solubility of DPH?
DPH is soluble in organic solvents like DMSO, ethanol, and chloroform but insoluble in water. It is typically dissolved in DMSO before being added to aqueous membrane suspensions.
How is DPH used in fluorescence anisotropy experiments?
DPH is incorporated into lipid membranes, and its fluorescence anisotropy is measured using a fluorometer. Changes in anisotropy reflect membrane fluidity and order.
What is the excitation and emission wavelength for DPH?
DPH is typically excited at around 360 nm and emits fluorescence at approximately 430 nm, though exact values may vary based on the solvent and experimental conditions.
Can DPH be used for live cell imaging?
While DPH is primarily used in in vitro membrane studies, it is not commonly used for live cell imaging due to its hydrophobic nature and potential toxicity at higher concentrations.
How should DPH be stored for long-term use?
Store DPH in a tightly sealed container at room temperature, protected from light and moisture. For extended storage, keeping it under an inert atmosphere (e.g., nitrogen) can prevent oxidation.
