Description
Specifications Table
| Product Name | 1M Tris Hydrochloride Buffer (1M Tris HCl) pH-8.0 |
|---|---|
| Pack Size | 100 ml, 500 ml |
| Form | Liquid Solution |
| Grade | Molecular Biology Grade |
| Application | DNA/RNA extraction, PCR, protein assays, electrophoresis |
Product Description
When precision matters in molecular biology, the right buffer can make all the difference. The 1M Tris Hydrochloride Buffer (1M Tris HCl) pH-8.0 from SRL Chem is a staple in labs across India, trusted by researchers, students, and educators for its consistency and reliability. Whether you’re running PCR, extracting nucleic acids, or stabilizing enzymes, this buffer ensures optimal pH conditions for your experiments.
Tris(hydroxymethyl)aminomethane, commonly known as Tris, is a widely used biological buffer due to its excellent pH buffering range (7.0–9.0). When combined with hydrochloric acid (HCl), it forms a stable solution that resists pH changes, even when diluted or exposed to temperature fluctuations. This makes it indispensable in protocols where pH stability is critical.
At eqipped, we understand the importance of high-quality reagents in achieving reproducible results. That’s why we source this buffer directly from SRL Chem, a brand synonymous with purity and performance in Indian laboratories. Whether you’re a student setting up your first gel electrophoresis or a researcher optimizing a new assay, this buffer is designed to meet your needs.
Need a reliable Tris HCl buffer for your molecular biology experiments? Buy 1M Tris HCl Buffer pH-8.0 online today and experience seamless performance in every run.
Why This Buffer Stands Out
Not all Tris buffers are created equal. This molecular biology-grade solution is free from DNase, RNase, and protease contaminants, ensuring it won’t interfere with sensitive downstream applications. The pH is precisely adjusted to 8.0, a sweet spot for many biochemical reactions, including DNA hybridization and protein purification.
For educators, this buffer is a great teaching tool. Its predictable behavior helps students grasp the fundamentals of buffering systems without the hassle of preparing solutions from scratch. Researchers appreciate its batch-to-batch consistency, which is crucial for long-term projects where variability can derail progress.
Common Applications
This versatile buffer finds use in a variety of molecular biology techniques:
- PCR and qPCR reactions
- DNA and RNA extraction protocols
- Agarose and polyacrylamide gel electrophoresis
- Protein purification and Western blotting
- Enzyme assays and biochemical reactions
Its compatibility with most laboratory equipment and protocols makes it a go-to choice for both routine and specialized applications.
Handling and Storage
To maintain the integrity of your buffer, store it at room temperature (15–25°C) in a tightly sealed container. Avoid repeated freeze-thaw cycles, as this can alter the pH. If you notice any precipitation or discoloration, do not use the solution, as this may indicate contamination or degradation.
Looking for a trusted supplier of Tris HCl buffer in India? Shop now at eqipped for genuine SRL Chem products delivered to your lab doorstep.
Risk Note
While Tris HCl is generally safe for laboratory use, it is important to handle it with care. Avoid skin and eye contact, and always wear appropriate personal protective equipment (PPE), such as gloves and goggles. In case of accidental exposure, rinse thoroughly with water and seek medical attention if irritation persists.
FAQs
What is the shelf life of 1M Tris HCl Buffer pH-8.0?
The buffer typically has a shelf life of 12–24 months when stored properly at room temperature. Always check the expiration date on the label before use.
Can I use this buffer for cell culture applications?
While Tris HCl is commonly used in molecular biology, it is not recommended for cell culture due to its potential toxicity to living cells. For cell culture, consider using HEPES or other biocompatible buffers.
How do I adjust the pH of this buffer if needed?
This buffer is pre-adjusted to pH 8.0. If you need a different pH, you can titrate it with HCl or NaOH, but we recommend using a pH meter for precise adjustments.
Is this buffer autoclavable?
Yes, Tris HCl buffer can be autoclaved at 121°C for 15–20 minutes to sterilize it. However, autoclaving may slightly alter the pH, so it’s best to check and adjust it post-sterilization if necessary.
Where can I buy 1M Tris HCl Buffer pH-8.0 online in India?
You can purchase this buffer from eqipped, where we offer genuine SRL Chem products with fast shipping across India. Order now to ensure your lab is always stocked with high-quality reagents.
