Description
Specifications Table
| Product Name | 2-Nitrophenyl-β-D-Galactopyranoside For | 8.41747.0001 | Merck |
|---|---|
| Quantity/Pack Size | 1 GM |
| Form | Powder |
| Grade | For Biochemistry |
| Application | β-Galactosidase substrate, ONPG assay, molecular biology research |
Product Description
2-Nitrophenyl-β-D-Galactopyranoside, commonly known as ONPG, is a synthetic substrate widely used in biochemical assays to detect β-galactosidase activity. This high-purity compound is essential for researchers studying gene expression, enzyme kinetics, and microbial metabolism. When hydrolyzed by β-galactosidase, ONPG releases a yellow-colored product (o-nitrophenol), allowing for easy quantification of enzymatic activity.
This product is particularly valuable in molecular biology laboratories for applications such as:
- LacZ gene expression studies
- Bacterial enzyme assays
- Protein purification monitoring
- Diagnostic test development
At eqipped, we ensure this ONPG substrate meets rigorous quality standards, making it suitable for both academic research and industrial applications. The 1g pack size offers flexibility for small-scale experiments while maintaining cost-effectiveness.
For reliable β-galactosidase assays, buy 2-Nitrophenyl-β-D-Galactopyranoside online from eqipped today.
FAQs
What is the molecular weight of 2-Nitrophenyl-β-D-Galactopyranoside?
The molecular weight of ONPG is approximately 301.25 g/mol.
How should I prepare a working solution of ONPG?
Dissolve the substrate in appropriate buffer (typically phosphate buffer) to achieve the desired concentration, usually between 1-4 mg/mL for standard assays.
What wavelength should I use to measure the reaction product?
The yellow o-nitrophenol product is typically measured at 420 nm using a spectrophotometer.
Can this substrate be used for quantitative assays?
Yes, ONPG is excellent for quantitative assays as the color intensity directly correlates with β-galactosidase activity when measured under controlled conditions.
What is the shelf life of this product?
When stored properly, 2-Nitrophenyl-β-D-Galactopyranoside maintains its stability for at least 2 years from the date of manufacture.
