Description
Specifications Table
| Product Name | 2,7-Dichlorofluorescein Diacetate (DCFH-DA) |
|---|---|
| Quantity/Pack Size | 25 Mg, 100 Mg |
| Form | Solid |
| Grade | Extrapure, 97% |
| Application | ROS detection, cellular assays |
Product Description
2,7-Dichlorofluorescein Diacetate (DCFH-DA) is a cell-permeable dye widely used for detecting reactive oxygen species (ROS) in live cells. Upon entering cells, it is hydrolyzed by intracellular esterases to form DCFH, which is then oxidized by ROS to produce fluorescent DCF. This makes it an essential tool for oxidative stress research, drug screening, and environmental toxicity studies.
With a purity of 97%, this extrapure grade ensures reliable and reproducible results in your experiments. Whether you’re a student conducting academic research or a professional in a lab setting, DCFH-DA from eqipped provides the consistency you need for accurate ROS measurements.
Need high-quality DCFH-DA for your research? Shop now at eqipped for guaranteed purity and fast delivery.
How DCFH-DA Works
DCFH-DA is non-fluorescent in its native state. Once inside cells, esterases cleave the acetate groups, converting it to DCFH. In the presence of ROS, DCFH is oxidized to highly fluorescent DCF, which can be quantified using fluorescence microscopy or flow cytometry. This mechanism makes it a versatile probe for studying oxidative stress in various biological systems.
Applications of DCFH-DA
DCFH-DA is commonly used in:
- Cellular oxidative stress assays
- Drug-induced toxicity studies
- Environmental pollutant research
- Neuroscience and aging studies
Looking for a trusted supplier of DCFH-DA in India? eqipped offers lab-grade reagents with fast shipping.
Handling and Storage
Store DCFH-DA at -20°C in a dry, dark place to prevent degradation. Avoid repeated freeze-thaw cycles. For best results, prepare fresh solutions before each experiment.
FAQs
What is the purity of this DCFH-DA?
This product is extrapure with a minimum purity of 97%, ensuring high sensitivity in ROS detection assays.
Can DCFH-DA be used in live-cell imaging?
Yes, DCFH-DA is cell-permeable and commonly used for real-time ROS detection in live cells.
What is the shelf life of DCFH-DA?
When stored properly at -20°C, DCFH-DA remains stable for up to 2 years.
Is DCFH-DA suitable for flow cytometry?
Absolutely. The fluorescent DCF produced after oxidation is detectable via flow cytometry.
Where can I buy DCFH-DA online in India?
You can purchase high-quality DCFH-DA from eqipped with fast delivery across India.
