Description
Specifications Table
| Product Name | 3-(2-Pyridyldithio)propionic Acid N-Hydroxysuccinimide Ester (SPDP Crosslinker) |
|---|---|
| Pack Size | 100 Mg – 1 Gms – 5 Gms |
| Form | Solid |
| Grade | Extrapure, 95% |
| Application | Protein conjugation, bioconjugation, sulfhydryl-reactive crosslinking |
Product Description
When precision matters in bioconjugation, the SPDP crosslinker stands out as a reliable choice for researchers and educators. This pyridyldithiopropionic acid derivative, combined with an NHS ester, offers a versatile tool for linking proteins, antibodies, and other biomolecules. Its sulfhydryl-reactive nature ensures efficient conjugation, making it indispensable in labs focusing on immunology, drug delivery, or diagnostic assays.
The extrapure grade (95%) guarantees minimal impurities, which is critical for sensitive applications. Whether you’re working on protein-protein conjugations or peptide labeling, this reagent provides consistent results. The NHS ester group reacts with primary amines, while the pyridyldithio moiety introduces a cleavable disulfide bond, allowing controlled release in reducing environments.
For students and researchers, understanding the mechanism of SPDP crosslinkers is key. The reagent first reacts with amine groups via the NHS ester, forming an amide bond. The pyridyldithio group then enables conjugation with sulfhydryl-containing molecules, creating stable yet reversible linkages. This dual functionality makes it a go-to reagent for antibody-drug conjugates (ADCs) and enzyme labeling.
At eqipped, we ensure that every batch meets rigorous quality standards, so you can focus on your experiments without worrying about reagent performance. Whether you’re ordering for a small-scale project or bulk research, our SPDP crosslinker delivers the purity and reactivity you need.
Upgrade your bioconjugation workflow with high-purity SPDP crosslinker. Buy online today for seamless protein conjugation.
FAQs
What is the shelf life of SPDP crosslinker?
When stored properly at -20°C, the SPDP crosslinker remains stable for up to 12 months. Always check the expiry date on the label.
Can SPDP crosslinker be used for in vivo applications?
While it is primarily used in vitro, some researchers have adapted it for in vivo studies. Consult literature or a specialist before use.
How does SPDP crosslinker compare to other NHS esters?
Unlike standard NHS esters, SPDP introduces a disulfide bond, allowing reversible conjugation under reducing conditions.
What is the ideal pH for SPDP conjugation?
The reaction works best at a pH of 7.2–8.5, ensuring optimal amine reactivity.
Is SPDP crosslinker compatible with all proteins?
It works well with most proteins containing primary amines or sulfhydryl groups. However, test compatibility for your specific application.
