Description
Specifications Table
Product Name – 3,3-DIAMINOBENZIDINE TETRAHYDROCHLORIDE 97% AR
Quantity/Pack Size – 1 g, 5 g
Form – Powder
Grade – Analytical Reagent (AR)
Application – Chromogenic substrate, immunohistochemistry, ELISA
Product Overview
3,3-Diaminobenzidine Tetrahydrochloride (DAB) at 97% AR grade is a highly purified chromogenic substrate widely recognized for its exceptional sensitivity and reliability in biochemical assays. This fine, off-white to light brown powder offers superior solubility in aqueous solutions, ensuring consistent performance in peroxidase-based detection systems. Its high analytical purity minimizes background noise, making it indispensable for applications requiring precise signal amplification, such as immunohistochemistry (IHC) and enzyme-linked immunosorbent assays (ELISA). The compound’s stable tetrahydrochloride form enhances shelf life while maintaining reactivity, even under stringent experimental conditions. Designed for researchers demanding reproducibility, this AR-grade DAB delivers sharp, high-contrast results without compromising specificity. Its compatibility with common buffers and fixation protocols further simplifies workflow integration, reducing variability across experiments. Whether used in manual staining or automated platforms, this product adheres to strict quality controls, guaranteeing lot-to-lot consistency for critical laboratory procedures.
FAQs
1. What is the solubility of 3,3-Diaminobenzidine Tetrahydrochloride in water?
It is freely soluble in water, typically forming a clear to slightly colored solution depending on concentration and pH.
2. Can this product be used with HRP-conjugated antibodies?
Yes, it is specifically optimized for horseradish peroxidase (HRP) systems, producing a brown precipitate upon oxidation.
3. How should this compound be stored for long-term stability?
Store in a tightly sealed container at 2–8°C, protected from light and moisture to prevent degradation.
4. Are there any known alternatives to DAB for peroxidase staining?
Alternatives include TMB (3,3′,5,5′-Tetramethylbenzidine) for soluble assays or AEC (3-Amino-9-Ethylcarbazole) for red staining, though DAB remains the gold standard for sensitivity.
5. Does this product require any special handling precautions?
Yes, it is classified as a potential carcinogen; use in a fume hood with appropriate PPE, including gloves and goggles.
