Description
Specifications Table
| Product Name | 3,3-Diaminobenzidine Tetrahydrochloride (DAB.4HCl) Buffer Substrate Solution for Peroxidase |
|---|---|
| Quantity/Pack Size | 100 ml |
| Form | Liquid |
| Grade | Laboratory Grade |
| Application | Peroxidase substrate for immunohistochemistry, ELISA, and Western blotting |
Product Description
When it comes to sensitive and reliable peroxidase-based assays, the right substrate makes all the difference. 3,3-Diaminobenzidine Tetrahydrochloride (DAB.4HCl) Buffer Substrate Solution is a trusted choice for researchers and lab professionals who need consistent, high-quality results in immunohistochemistry (IHC), ELISA, and Western blotting.
This ready-to-use buffer substrate solution eliminates the need for time-consuming preparation, ensuring you get accurate and reproducible staining every time. Whether you’re working in a research lab, diagnostic center, or educational institution, this DAB.4HCl solution provides the clarity and precision required for critical experiments.
Why is DAB.4HCl the preferred substrate for peroxidase assays? Unlike other substrates, DAB.4HCl produces a stable, insoluble brown precipitate that’s easy to visualize under a microscope. This makes it ideal for detecting horseradish peroxidase (HRP)-conjugated antibodies in tissue sections, cell cultures, and protein blots.
At eqipped, we understand the importance of reliable lab supplies. That’s why we source this DAB.4HCl buffer substrate solution from trusted manufacturers like SRL Chem, ensuring you receive a product that meets stringent quality standards. Whether you’re a student learning immunohistochemistry techniques or a seasoned researcher refining your protocols, this solution delivers the performance you need.
Looking for a dependable peroxidase substrate for your next experiment? Order your DAB.4HCl buffer substrate solution today and experience the difference in your results.
FAQs
What is the shelf life of DAB.4HCl buffer substrate solution?
The shelf life is typically 12–24 months when stored properly at 2–8°C. Always check the expiration date on the label before use.
Can I use this DAB.4HCl solution for both manual and automated staining?
Yes, this buffer substrate solution is compatible with both manual staining protocols and automated immunohistochemistry systems.
Is this DAB.4HCl solution ready to use, or does it require dilution?
This solution is ready to use and does not require additional dilution. However, always verify the manufacturer’s instructions for your specific application.
What is the difference between DAB and DAB.4HCl?
DAB.4HCl is the tetrahydrochloride salt of 3,3-Diaminobenzidine, which enhances its solubility and stability in aqueous solutions, making it more suitable for laboratory use.
Can I use this substrate for alkaline phosphatase (AP) assays?
No, DAB.4HCl is specifically designed for peroxidase (HRP) assays. For alkaline phosphatase, consider substrates like BCIP/NBT or Fast Red.
