Description
Specifications Table
| Product Name | 3,3,5,5-Tetramethylbenzidine (TMB) extrapure AR |
|---|---|
| Pack Size | 1 Gms, 5 Gms |
| Form | Solid |
| Grade | Extrapure AR, ExiPlus, Multi-Compendial |
| Application | ELISA, biochemical assays, research |
Product Description
When precision matters in your lab, 3,3,5,5-Tetramethylbenzidine (TMB) extrapure AR stands out as the gold standard for ELISA and biochemical applications. This high-purity reagent, certified to meet BP and Ph.Eur compendial specifications, ensures reliable results in immunoassays, enzyme studies, and diagnostic testing. With a guaranteed 98% purity and ExiPlus certification, it minimizes background noise and maximizes sensitivity—critical for accurate quantitative analysis.
Researchers and educators trust TMB extrapure AR for its consistency and stability. Whether you’re developing diagnostic kits or conducting academic research, this grade delivers sharp colorimetric responses, making it ideal for peroxidase-based assays. The extrapure designation means fewer impurities, reducing variability in your experiments.
At eqipped, we source this TMB variant directly from SRL Chem, ensuring you receive authentic, lab-tested material. Available in convenient 1g and 5g packs, it’s perfect for both small-scale trials and bulk experiments. Need TMB extrapure for your next ELISA project? Order now and experience the difference in quality.
Why TMB Extrapure AR is Essential for Your Lab
ELISA assays demand reagents that won’t compromise your data. Here’s why this TMB grade is a top choice:
First, its multi-compendial compliance guarantees adherence to strict pharmaceutical standards, so your results align with global benchmarks. The ExiPlus certification adds another layer of assurance, confirming it meets rigorous purity criteria for analytical applications.
Second, the 98% purity level eliminates common interferences like heavy metals or organic contaminants, which can skew absorbance readings. This is especially valuable in low-concentration assays where sensitivity is paramount.
Finally, its versatility extends beyond ELISA. Use it in Western blot development, enzyme kinetics studies, or even as a substrate in histochemistry. The stable, soluble nature of TMB extrapure AR makes it adaptable to various protocols without sacrificing performance.
Applications in Research and Education
From university labs to R&D centers, TMB extrapure AR finds use in:
1. ELISA and Immunoassays: The cornerstone of diagnostic testing, where TMB’s blue-to-yellow color shift provides clear, quantifiable results.
2. Biochemical Research: Studying enzyme activity, particularly horseradish peroxidase (HRP), where TMB acts as a highly sensitive substrate.
3. Educational Demonstrations: Ideal for teaching colorimetric techniques, thanks to its predictable and vivid reactions.
4. Clinical Diagnostics: Used in kits for detecting hormones, antibodies, and infectious agents with high specificity.
Looking for a reliable TMB extrapure supplier? eqipped delivers lab-grade chemicals with verified purity—shop now for seamless procurement.
Handling and Storage Guidelines
To maintain the integrity of your TMB extrapure AR:
Store in a cool, dry place away from direct sunlight. The recommended temperature range is 2–8°C for long-term stability. Always use chemically resistant gloves and work in a well-ventilated area or fume hood, as TMB is a potential irritant.
Avoid moisture exposure, as it can degrade the compound. For best results, prepare fresh solutions before each assay and discard unused portions to prevent contamination.
Safety Considerations
Risk Note: TMB is classified as harmful if inhaled or swallowed. It may cause skin and eye irritation. Wear appropriate PPE (gloves, goggles, lab coat) and follow standard lab safety protocols. In case of contact, rinse immediately with plenty of water and seek medical advice. Dispose of waste according to local chemical safety regulations.
FAQs
What is the difference between TMB extrapure AR and regular TMB?
Extrapure AR grade meets stricter compendial standards (BP, Ph.Eur) and undergoes additional purification to remove trace impurities, ensuring higher sensitivity in assays compared to standard grades.
Can I use this TMB for quantitative ELISA?
Absolutely. The 98% purity and ExiPlus certification make it ideal for quantitative ELISA, providing consistent absorbance values for accurate concentration measurements.
How should I prepare a TMB solution for ELISA?
Dissolve TMB in DMSO or a suitable buffer (e.g., citrate buffer pH 5.0) to a concentration of 1 mg/mL. Filter sterilize if needed and use immediately for optimal results.
Is this TMB suitable for Western blotting?
Yes, it’s commonly used as an HRP substrate in Western blots, offering high sensitivity and low background when paired with appropriate detection systems.
Where can I buy TMB extrapure AR in India?
You can buy TMB extrapure AR online from eqipped, with fast shipping across India. We ensure authentic SRL Chem products with verified purity.
