4-Fluoro-7-nitrobenzofurazan, 98% 5MG

Thermo Fisher Scientific Chemicals Dealer | eqipped

4-Fluoro-7-nitrobenzofurazan, 98% 5MG

Ultra-pure 4-Fluoro-7-Nitrobenzofurazan (98% grade) for precise fluorescence labeling. High reactivity, low impurities, ideal for biochemical assays.

Original price was: ₹45,544.00.Current price is: ₹31,881.00.

Specifications Table

Product Name – 4-Fluoro-7-nitrobenzofurazan Quantity/Pack Size – 5MG Form – Solid (Powder/Crystals) Grade – 98% (HPLC) Application – Fluorescent labeling, protein modification, biochemical research

Product Overview

4-Fluoro-7-nitrobenzofurazan (NBD-F) is a highly reactive fluorogenic reagent widely recognized for its exceptional selectivity in labeling primary and secondary amines. With a purity of 98% as confirmed by HPLC analysis, this compound ensures minimal background interference, making it indispensable for applications requiring high sensitivity. The molecule’s unique nitrobenzofurazan structure enables strong fluorescence upon conjugation, with excitation and emission maxima typically observed in the visible range (460-540 nm), facilitating easy detection in biochemical assays. Its stability under standard lab conditions and compatibility with aqueous and organic solvents enhance its versatility. The 5MG pack size is optimized for small-scale experiments, reducing waste while maintaining cost-efficiency. This reagent is particularly valued for its rapid reaction kinetics, often completing labeling within minutes under mild conditions (pH 7-9), without requiring harsh catalysts. The absence of heavy metal contaminants and low moisture content (<0.5%) further ensures reproducibility in critical applications. Researchers benefit from its consistent performance across diverse protocols, from protein quantification to enzyme activity studies. The compound’s shelf life of 24 months when stored at -20°C in a desiccated environment underscores its reliability for long-term lab inventory.

FAQs

1. What solvents are compatible with 4-Fluoro-7-nitrobenzofurazan for dissolution?

It dissolves readily in dimethyl sulfoxide (DMSO), dimethylformamide (DMF), and acetone. Avoid prolonged exposure to water to prevent hydrolysis.

2. Can this reagent be used for labeling peptides with internal amines?

Yes, but reactivity may vary based on steric hindrance. Pre-testing with model compounds is recommended for optimal results.

3. What alternatives exist for non-fluorescent amine labeling?

Consider sulfosuccinimidyl esters (e.g., Sulfo-NHS) for non-fluorescent applications, though they lack the detection sensitivity of NBD-F.

4. How should unused reagent be stored to maintain stability?

Store in a tightly sealed amber vial at -20°C, protected from light and moisture. Use desiccant packs to absorb residual humidity.

5. Is this compound suitable for in vivo imaging studies?

No, its cytotoxicity and limited tissue penetration make it unsuitable for in vivo applications. Restrict use to in vitro or ex vivo protocols.

Thermo Fisher Scientific Chemicals Dealer | eqipped

4-Fluoro-7-nitrobenzofurazan, 98% 5MG

Original price was: ₹45,544.00.Current price is: ₹31,881.00.

Ultra-pure 4-Fluoro-7-Nitrobenzofurazan (98% grade) for precise fluorescence labeling. High reactivity, low impurities, ideal for biochemical assays.

Specifications Table

Product Name – 4-Fluoro-7-nitrobenzofurazan Quantity/Pack Size – 5MG Form – Solid (Powder/Crystals) Grade – 98% (HPLC) Application – Fluorescent labeling, protein modification, biochemical research

Product Overview

4-Fluoro-7-nitrobenzofurazan (NBD-F) is a highly reactive fluorogenic reagent widely recognized for its exceptional selectivity in labeling primary and secondary amines. With a purity of 98% as confirmed by HPLC analysis, this compound ensures minimal background interference, making it indispensable for applications requiring high sensitivity. The molecule’s unique nitrobenzofurazan structure enables strong fluorescence upon conjugation, with excitation and emission maxima typically observed in the visible range (460-540 nm), facilitating easy detection in biochemical assays. Its stability under standard lab conditions and compatibility with aqueous and organic solvents enhance its versatility. The 5MG pack size is optimized for small-scale experiments, reducing waste while maintaining cost-efficiency. This reagent is particularly valued for its rapid reaction kinetics, often completing labeling within minutes under mild conditions (pH 7-9), without requiring harsh catalysts. The absence of heavy metal contaminants and low moisture content (<0.5%) further ensures reproducibility in critical applications. Researchers benefit from its consistent performance across diverse protocols, from protein quantification to enzyme activity studies. The compound’s shelf life of 24 months when stored at -20°C in a desiccated environment underscores its reliability for long-term lab inventory.

FAQs

1. What solvents are compatible with 4-Fluoro-7-nitrobenzofurazan for dissolution?

It dissolves readily in dimethyl sulfoxide (DMSO), dimethylformamide (DMF), and acetone. Avoid prolonged exposure to water to prevent hydrolysis.

2. Can this reagent be used for labeling peptides with internal amines?

Yes, but reactivity may vary based on steric hindrance. Pre-testing with model compounds is recommended for optimal results.

3. What alternatives exist for non-fluorescent amine labeling?

Consider sulfosuccinimidyl esters (e.g., Sulfo-NHS) for non-fluorescent applications, though they lack the detection sensitivity of NBD-F.

4. How should unused reagent be stored to maintain stability?

Store in a tightly sealed amber vial at -20°C, protected from light and moisture. Use desiccant packs to absorb residual humidity.

5. Is this compound suitable for in vivo imaging studies?

No, its cytotoxicity and limited tissue penetration make it unsuitable for in vivo applications. Restrict use to in vitro or ex vivo protocols.

My Cart
Recently Viewed
Categories

Join Eqipped Circle

By Clicking “Join Now”, you are confirming that you have read and agree to eqipped.com ‘s Terms of Use and Privacy Policy.

Collaborate With Us

By Clicking “Download Now”, you are confirming that you have read and agree to eqipped.com ‘s Terms of Use and Privacy Policy.

💬
Eqipped

Get Price and available Brands of Product, I’d love to help you 😊

×