Description
Specifications Table
| Product Name | 5-Sulphosalicylic Acid 20% Solution | 894670 | CDH |
|---|---|
| Pack Size | 125 ml – 250 ml |
| Form | Liquid |
| Grade | AR (Analytical Reagent) |
| Application | Protein precipitation – Metal ion detection – Biochemical assays |
Product Description
When it comes to protein analysis in laboratories, having the right reagents is crucial for accurate results. The 5-Sulphosalicylic Acid 20% Solution from CDH is a trusted choice among researchers, students, and educators for protein precipitation. This ready-to-use solution simplifies the process, ensuring consistent performance in biochemical assays.
This AR grade chemical is widely used in qualitative and quantitative protein estimation methods. Unlike solid forms, the pre-dissolved solution saves time and eliminates the need for manual preparation, reducing errors in concentration. Whether you are working on a university project or conducting routine lab tests, this reagent provides the precision you need.
Many labs across India rely on this solution for its stability and effectiveness in precipitating proteins without denaturing them. The 20% concentration is optimized for standard protocols, making it a staple in biochemistry and clinical chemistry setups. Buy 5 sulphosalicylic acid solution online from eqipped for fast delivery and competitive pricing.
Applications in Laboratory Settings
Protein precipitation is a fundamental step in many biochemical workflows. The 5-sulphosalicylic acid solution serves multiple purposes in a lab environment.
In clinical diagnostics, it helps isolate proteins from serum or urine samples for further analysis. Research labs use it in protein purification processes where selective precipitation is required. Educational institutions also prefer this reagent for its ease of use in student experiments, allowing learners to focus on the methodology rather than reagent preparation.
Another advantage is its compatibility with spectroscopic methods. The precipitated proteins can be easily quantified using UV-Vis spectroscopy, a common technique in biochemistry. This makes the solution ideal for both teaching and advanced research applications.
Why This Solution Stands Out
Not all reagent solutions are created equal. The CDH 5-Sulphosalicylic Acid 20% Solution stands out due to its consistent quality and long shelf life when stored properly. The aqueous form ensures uniform distribution of the acid, which is critical for reproducible results.
For labs that handle multiple samples daily, this solution reduces preparation time significantly. It also minimizes the risk of exposure to solid powder, which can be hazardous if inhaled. The pre-mixed solution is a safer and more convenient option for routine use.
In addition to protein studies, this reagent finds applications in the detection of iron and other metal ions, making it a versatile addition to any chemical inventory. Looking for a reliable 5 sulphosalicylic acid supplier? eqipped offers genuine CDH products.
Handling and Storage Recommendations
While the solution is stable under normal conditions, proper storage extends its usability. Always keep the bottle tightly sealed and store it in a cool, dry place away from direct sunlight. This prevents degradation and maintains the reagent’s effectiveness over time.
When handling the solution, use standard lab safety gear, including gloves and goggles. Although the 20% concentration is less hazardous than the solid form, it can still cause irritation upon contact with skin or eyes. In case of accidental exposure, rinse the affected area immediately with plenty of water.
For long-term storage, check the bottle for any signs of crystallization or color change before use. If the solution appears cloudy or contains particles, it may indicate contamination or degradation, and a fresh batch should be used.
Common Experimental Protocols
One of the most frequent uses of this solution is in the precipitation of serum proteins. A typical protocol involves mixing a small volume of the solution with the sample, followed by centrifugation to separate the precipitated proteins. The clarity of the supernatant can then be measured to determine protein concentration.
In educational settings, this method is often demonstrated to students as part of protein estimation experiments. The simplicity of the procedure makes it an excellent teaching tool for understanding the principles of protein chemistry.
Researchers working on enzyme studies also use this reagent to remove interfering proteins before analyzing enzyme activity. The precipitated proteins can be redissolved later if needed, making this a reversible process in many cases.
Comparing with Alternatives
Some labs may consider using trichloroacetic acid or acetone for protein precipitation. However, 5-sulphosalicylic acid offers distinct advantages. It is less volatile than acetone, reducing health risks in the lab. Compared to trichloroacetic acid, it produces a more complete precipitation with fewer artifacts.
The 20% solution is particularly beneficial forhigh-throughput applications where speed and consistency are essential. Unlike solid reagents that require weighing and dissolving, this liquid form ensures that each experiment starts with the same concentration, eliminating variability.
For labs that prioritize safety, this solution is a better choice than handling powdered 5-sulphosalicylic acid, which can be irritating to the respiratory system. The liquid form reduces dust exposure, making it a preferred option in many research facilities.
Who Should Use This Product?
This reagent is suitable for a wide range of users. University students working on biochemistry projects will find it easy to incorporate into their experiments. College labs stock this solution for routine protein analysis demonstrations.
Research institutions and diagnostic centers use it for its reliability in clinical and biochemical assays. Even small-scale private labs benefit from its long shelf life and ease of use.
Educators appreciate the solution’s role in teaching fundamental biochemical techniques. Its consistent performance allows students to focus on learning rather than troubleshooting reagent issues.
FAQs
What is the primary use of 5-sulphosalicylic acid solution?
It is primarily used for precipitating proteins in biochemical and clinical laboratory settings, making it easier to analyze protein content in samples.
How should I store the 5-sulphosalicylic acid 20% solution?
Store the solution in a cool, dry place with the bottle tightly sealed to prevent contamination or degradation.
Is this solution safe for student lab experiments?
Yes, when used with proper safety precautions like gloves and goggles, it is safe for educational purposes under supervision.
Can I use this solution for iron detection?
Yes, 5-sulphosalicylic acid is also effective in detecting iron and other metal ions in qualitative analysis.
Where can I buy 5 sulphosalicylic acid solution online in India?
You can buy it from eqipped, a trusted supplier of lab chemicals and reagents with fast delivery across India.
