Description
Specifications Table
| Product Name | 5,5′-Dithiobis(2-Nitro Benzoic Acid) extrapure (DTNB), 98% (Ellman’s Reagent) |
|---|---|
| Pack Size | 5 Gms, 25 Gms, 100 Gms |
| Form | Powder |
| Grade | Extrapure |
| Application | Thiol quantification, protein assays, biochemical research |
Product Description
When precision matters in your lab, 5,5′-Dithiobis(2-Nitro Benzoic Acid) (DTNB), commonly known as Ellman’s Reagent, is the gold standard for quantifying thiol groups in proteins and other biomolecules. This extrapure grade DTNB, with 98% purity, ensures accurate and reproducible results in your research or diagnostic applications.
Whether you’re a student working on a biochemistry project or a researcher developing new assays, this high-quality DTNB reagent from SRL Chem provides the reliability you need. The bright yellow color of the reagent upon reaction with thiols makes it easy to measure absorbance at 412 nm, simplifying your workflow.
At eqipped, we understand the importance of using top-tier reagents in your experiments. That’s why we source this DTNB directly from trusted manufacturers to guarantee consistency batch after batch. Need DTNB for your next experiment? Buy online now and get it delivered to your lab doorstep across India.
Ellman’s Reagent is widely used in:
- Protein quantification assays
- Enzyme activity studies
- Drug development research
- Diagnostic kit manufacturing
The extrapure grade ensures minimal interference from impurities, making it ideal for sensitive applications. Each pack comes in convenient sizes (5g, 25g, or 100g) to match your lab’s consumption needs.
Remember, proper handling is crucial for this chemical. Always store in a cool, dry place away from direct sunlight, and follow standard lab safety protocols when using DTNB.
FAQs
1. What is DTNB used for in laboratories?
DTNB, or Ellman’s Reagent, is primarily used to quantify thiol groups (-SH) in proteins and other biomolecules. It’s essential for protein assays, enzyme activity studies, and research involving sulfur-containing compounds.
2. How do I prepare a DTNB solution for my assay?
Dissolve DTNB in a suitable buffer (typically 0.1 M phosphate buffer, pH 8.0) to make a 4 mg/mL stock solution. Filter if necessary and store protected from light. Always prepare fresh solutions for best results.
3. What’s the difference between regular and extrapure DTNB?
Extrapure DTNB undergoes additional purification steps to remove trace impurities, making it ideal for sensitive applications where background interference could affect results. It’s the preferred choice for research and diagnostic use.
4. How should I store DTNB to maintain its stability?
Store DTNB in its original container, tightly sealed, at 2-8?C. Protect from light and moisture. Properly stored, it maintains stability for at least 2 years from manufacture date.
5. Can I buy DTNB in bulk quantities from eqipped?
Yes, eqipped offers DTNB in 5g, 25g, and 100g pack sizes. For larger research needs, contact our customer support for bulk pricing and availability.
