Specifications Table

Product Name – 9-Fluorenylmethyl chloroformate
Quantity/Pack Size – 5GR
Form – Solid (Crystalline Powder)
Grade – 98% (HPLC)
Application – Peptide synthesis, amino acid protection, protein sequencing

Product Overview

9-Fluorenylmethyl chloroformate (Fmoc-Cl) is a high-purity, lab-grade reagent essential for solid-phase peptide synthesis (SPPS) and biochemical research. With a minimum assay of 98% (HPLC), this crystalline powder ensures reliable Fmoc protection of amino groups, enabling efficient peptide chain elongation with minimal side reactions. Its low moisture content (<0.5%) and high reactivity make it ideal for sensitive applications where precision is critical. The compound’s stability under standard lab conditions (when stored at 2–8°C in a desiccated environment) prevents premature degradation, while its compatibility with common organic solvents like DMF, DCM, and acetonitrile allows seamless integration into existing synthesis protocols. Unlike alternative protecting groups, Fmoc-Cl offers orthogonal deprotection under mild basic conditions (e.g., piperidine), reducing risk to labile functional groups. This reagent is particularly valued for its role in automated peptide synthesizers, where consistency and reproducibility are paramount. Equipped’s 9-Fluorenylmethyl chloroformate undergoes rigorous quality control to eliminate impurities like fluorenylmethanol or residual acids, ensuring batch-to-batch reliability for high-stakes research.

FAQs

1. What is the shelf life of 9-Fluorenylmethyl chloroformate when stored properly?

When stored at 2–8°C in a tightly sealed container with desiccant, the reagent retains ≥98% purity for 24 months from the date of manufacture. Avoid exposure to humidity or prolonged room temperature storage.

2. Can this reagent be used for N-terminal protein sequencing?

Yes, Fmoc-Cl is compatible with Edman degradation-based sequencing due to its selective reactivity with primary amines. However, optimize reaction conditions (pH, solvent) to minimize side reactions with lysine or cysteine residues.

3. What solvents are recommended for dissolving Fmoc-Cl?

Dry acetonitrile, DMF, or dichloromethane (DCM) are ideal solvents. Avoid protic solvents like methanol or water, which can hydrolyze the chloroformate group and reduce yield.

4. How does Fmoc-Cl compare to Boc anhydride for amino protection?

Fmoc-Cl offers orthogonal deprotection (base-labile) versus Boc’s acid-labile cleavage, making it preferable for SPPS where acidic conditions could damage acid-sensitive residues. Fmoc also enables real-time monitoring via UV absorbance (301 nm).

5. What safety precautions should be taken when handling this compound?

Fmoc-Cl is a potent irritant and sensitizer. Handle in a fume hood with nitrile gloves, safety goggles, and lab coat. In case of skin contact, wash immediately with soap and water. Neutralize spills with sodium bicarbonate solution before disposal.